Protein Info for DZA65_RS20260 in Dickeya dianthicola ME23

Annotation: bifunctional isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 PF20423: AceK_regulatory" amino acids 10 to 306 (297 residues), 371.2 bits, see alignment E=4e-115 PF06315: AceK_kinase" amino acids 311 to 564 (254 residues), 407.5 bits, see alignment E=2.4e-126

Best Hits

Swiss-Prot: 83% identical to ACEK_PECCP: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 97% identity to dze:Dd1591_0367)

MetaCyc: 76% identical to isocitrate dehydrogenase kinase/phosphatase (Escherichia coli K-12 substr. MG1655)
3.1.3.-; [Isocitrate dehydrogenase (NADP(+))] kinase. [EC: 2.7.11.5]

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CDW3 at UniProt or InterPro

Protein Sequence (585 amino acids)

>DZA65_RS20260 bifunctional isocitrate dehydrogenase kinase/phosphatase (Dickeya dianthicola ME23)
MSRGREQLVAQTILQGFDAQYGRFLEVTAGAQQRFEQADWHAVQQAMKQRIQLYDHHVGL
VVEQLGCITDRQCYDAAFVARVKQIYTALLPDYPRFEIAESFFNSVYCRLFHHRELTPDK
LFVFSSQPARRFQEIPRPLARTYSPDNGWPAMLEAVLTALPLRLPWEDLTRDIGYVVRSL
REAFPAASLANVSLQVANELFYRNKAAWLVGKLRLPGGVFPFLLPIHHNERGALFIDTCL
TRHADASIVFGFARSYFMVYAPLPSALVAWLREILPGKTTAELYLAIGCQKHSKTEYYRE
YLDFIAGTQEPFITAPGVKGMVMLVFTLPTFDRVFKVIKDRFAPQKEVSEARVRECYRLV
KEHDRVGRMADTQEYENFVLEKARISPELLAELWREVPDKLEDLGDRLVIRHLYMERRMT
PLNLYLEQASGQALRDVIDEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPPRDPADELAAEPWYSVGPNDVFPEEFRQFLCSDRRLLPLFDELHGDLF
RAEYWRALQQRIRSGYIDDVYAYRRRKRFSLRYADPVTPADALTS