Protein Info for DZA65_RS20165 in Dickeya dianthicola ME23

Annotation: miniconductance mechanosensitive channel MscM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1107 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 469 to 494 (26 residues), see Phobius details amino acids 518 to 543 (26 residues), see Phobius details amino acids 557 to 581 (25 residues), see Phobius details amino acids 603 to 621 (19 residues), see Phobius details amino acids 633 to 653 (21 residues), see Phobius details amino acids 674 to 696 (23 residues), see Phobius details amino acids 702 to 722 (21 residues), see Phobius details amino acids 789 to 811 (23 residues), see Phobius details amino acids 832 to 852 (21 residues), see Phobius details amino acids 873 to 898 (26 residues), see Phobius details amino acids 906 to 933 (28 residues), see Phobius details PF12795: MscS_porin" amino acids 32 to 259 (228 residues), 183 bits, see alignment E=1.8e-57 PF12794: MscS_TM" amino acids 482 to 818 (337 residues), 345.2 bits, see alignment E=8.4e-107 PF21088: MS_channel_1st" amino acids 879 to 920 (42 residues), 38.5 bits, see alignment (E = 2.2e-13) PF00924: MS_channel_2nd" amino acids 922 to 987 (66 residues), 86.5 bits, see alignment 2.7e-28 PF21082: MS_channel_3rd" amino acids 995 to 1076 (82 residues), 39.8 bits, see alignment 1.3e-13

Best Hits

Swiss-Prot: 67% identical to MSCM_ECOLI: Miniconductance mechanosensitive channel MscM (mscM) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 97% identity to ddd:Dda3937_02149)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CNK8 at UniProt or InterPro

Protein Sequence (1107 amino acids)

>DZA65_RS20165 miniconductance mechanosensitive channel MscM (Dickeya dianthicola ME23)
MRLILIFLLGCLLSTTSLAAQLPDETQLKQDLQQAETNQTSPAQADIVKELQSALRLLDE
RRDTRQRADQYQRAIDDFPKLTRDLRQQLDAENAKPAAPPKATSVNDLEQQIVQLSSQLL
EQSRQLQQEQDHQREISDSLAQLPQQQTEANRALSEVERRVQALGNPTTALGQAQLAARQ
ADAALRKSRVEELELAQLSASNRQELSRLQADVYKKRRDRLDNQLQLLRSALNTLRQREA
EQALERTEQLAEQEGQLPTAVAGLLQTNRELSIALNQQAQLMDQIAARQRQTAAQTLQVR
QALSTLREQAQWLGSSPALGETLRAQVARLPEMPKPQQLDGDMAQLRAQRLHYEDLLNKL
SATGEPARQDDGAPLTAAQQKIVNDQQRTQRDLLTSLISGCDTQILELTKLKVASSQLED
ALTEIRDAAHRYLFWVADVDPIGFAYPLNLLRDLSRLLSLDTLTQLSGAMLMMATSQSTL
LPLLAALLLVGLSISSRRHYHAFLERASSRVGKVTLDYFMLTLRTVFWSVMVAIPLPVLW
AALGYGLQNAWPYPVAVAIGDSVTATVPLMWVVMICAAFSHRQGLFIVHFGWSPKQVARA
MRYYRLSIGLIVPLVMALITFDNLNDREFSSTLGRLCFILLCMALSLVTTSLKRAGIPLY
LDKEGSGENPVNRAMCNLMICIPLIAALASCLGYLATSQALLARLETSVAIWFFLLVIYH
IIRRWMWIQRRRIAFDRARQRRADMLAQRARGEEDASPFSHEAGVDVVEEPVVDLDAISA
RSLKLVRSILTLIALMSVIALWSEIHSAFAFMENISLWDVTSTVKGVESVQPITLGAVLI
ALLVFVITAQLVRNLPALLELAVLQHIELSPGTGYAIITVSKYLMMLVGGLMGFSLIGIE
WSKLQWLVAALGVGLGFGLQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVMRINTRA
TTISDWDRKEIIVPNKAFITEQFINWSLSDSVTRVVLTIPAPADASTQQVTTLLLDAVKR
CSLVLDNPPPEVFLVDLRQGIQIFELRIFAAEMGHRMPLRHELHQLILESYREHNLVMPF
PPFQVQMDSVQIAGRNAATSTRTAGGL