Protein Info for DZA65_RS20025 in Dickeya dianthicola ME23

Annotation: nitrogenase molybdenum-iron protein alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 482 TIGR01282: nitrogenase molybdenum-iron protein alpha chain" amino acids 12 to 478 (467 residues), 848.6 bits, see alignment E=1.2e-259 TIGR01862: nitrogenase component I, alpha chain" amino acids 27 to 472 (446 residues), 621.1 bits, see alignment E=9.8e-191 PF00148: Oxidored_nitro" amino acids 62 to 466 (405 residues), 322.9 bits, see alignment E=1.5e-100

Best Hits

Swiss-Prot: 90% identical to NIFD_KLEPN: Nitrogenase molybdenum-iron protein alpha chain (nifD) from Klebsiella pneumoniae

KEGG orthology group: K02586, nitrogenase molybdenum-iron protein alpha chain [EC: 1.18.6.1] (inferred from 98% identity to ddd:Dda3937_02156)

MetaCyc: 90% identical to [FeMo]-nitrogenase complex dinitrogenase component alpha subunit (Klebsiella pneumoniae)
Nitrogenase (flavodoxin). [EC: 1.19.6.1]

Predicted SEED Role

"Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1 or 1.19.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4C609 at UniProt or InterPro

Protein Sequence (482 amino acids)

>DZA65_RS20025 nitrogenase molybdenum-iron protein alpha chain (Dickeya dianthicola ME23)
MTNATSERNQEIIQEVLEIFPEKARKERKKHMMVTDPSMDGVGKCIVSNRKSQPGVMTVR
GCAYAGSKGVVFGPIKDMAHISHGPIGCGQYSRAGRRNYYTGVSGVNSFGTLNFTSDFQE
KDIVFGGDKKLTKLIEELELLFPLTKGISIQSECPVGLIGDDIEAVANASRKAIGKPVVP
VRCEGFRGVSQSLGHHIANDVIRDWVLDNREGKPFESTPYDVAIIGDYNIGGDAWASRIL
LEEMGLRVVAQWSGDGTLVEMENTPHVKLNLVHCYRSMNYISRHMEEKHGIPWMEYNFFG
PTKIAESLRKIAAQFDETIQNNAEAVIARYQAQTDAVIAKYRPRLEGRKVLLYLGGLRPR
HIIGAYEDLGMEIIGTGYEFGHNDDYDRTLPELKEGTLLFDDASSYELEAFVKALKPDLI
GSGIKEKYIFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMTLNNPSWSELTAPWLK
SA