Protein Info for DZA65_RS19890 in Dickeya dianthicola ME23

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 transmembrane" amino acids 433 to 452 (20 residues), see Phobius details TIGR01891: amidohydrolase" amino acids 21 to 434 (414 residues), 339.1 bits, see alignment E=1.7e-105 PF01546: Peptidase_M20" amino acids 81 to 269 (189 residues), 35.6 bits, see alignment E=9.5e-13 PF07687: M20_dimer" amino acids 189 to 280 (92 residues), 24.6 bits, see alignment E=2.1e-09

Best Hits

Swiss-Prot: 65% identical to ABGB_ECOLI: p-aminobenzoyl-glutamate hydrolase subunit B (abgB) from Escherichia coli (strain K12)

KEGG orthology group: K12941, aminobenzoyl-glutamate utilization protein B (inferred from 97% identity to ddd:Dda3937_02187)

MetaCyc: 65% identical to p-aminobenzoyl-glutamate hydrolase subunit B (Escherichia coli K-12 substr. MG1655)
3.5.1.-

Predicted SEED Role

"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit B" in subsystem p-Aminobenzoyl-Glutamate Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y251 at UniProt or InterPro

Protein Sequence (483 amino acids)

>DZA65_RS19890 amidohydrolase (Dickeya dianthicola ME23)
MTQTIVEFVNDMLAARAQPLAAIADDIWDHPETRFTEHYSAERLAGVLEAEGFSVRRGIA
GIDTAFVASFGSGQPVVALLGEFDALAGLSQQAGCAAPAPLTAGGNGHGCGHNLLGTAGL
GGVLAVKAWLEQHPQAGTVRFYGCPGEEGGSGKTFMAREGVFDDVDAALTWHPETFSGMF
CTPTLANIQAAFRFKGVAAHAAAAPHLGRSALDAVTLMNTGANFLREHIIPEARLHYAVT
DAGGHSPNVVQADAEVLYLVRAPQIDQAQAIYERVVNIARGAALMTDTTLTVRFDKACSN
YVPNRTLEQAMYGFVQAFGVPAYSEQEQTYAADIVRTLSAEDIASNLSSIARTGGAAGQA
FSRSLKGHALTSQVAPYAPTDALLSGSTDVGDVSWLVPTAQCFSPCYALGTPLHTWQAVA
QGRTSLAHKGMLLAAKVMSATALALFTDSALLQACRQEWRQQRQETPYRCPIPAGIQPSP
LQG