Protein Info for DZA65_RS19730 in Dickeya dianthicola ME23
Annotation: GDP-mannose 4,6-dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 84% identical to GM4D_VIBCH: GDP-mannose 4,6-dehydratase (gmd) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
KEGG orthology group: K01711, GDPmannose 4,6-dehydratase [EC: 4.2.1.47] (inferred from 98% identity to ddd:Dda3937_00444)MetaCyc: 84% identical to GDP-D-mannose 4,6-dehydratase (Vibrio cholerae O1)
GDP-mannose 4,6-dehydratase. [EC: 4.2.1.47]
Predicted SEED Role
"GDP-mannose 4,6-dehydratase (EC 4.2.1.47)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 4.2.1.47)
MetaCyc Pathways
- colanic acid building blocks biosynthesis (11/11 steps found)
- GDP-L-fucose biosynthesis I (from GDP-D-mannose) (3/3 steps found)
- GDP-6-deoxy-D-talose biosynthesis (1/2 steps found)
- GDP-D-perosamine biosynthesis (1/2 steps found)
- GDP-D-rhamnose biosynthesis (1/2 steps found)
- GDP-L-colitose biosynthesis (3/5 steps found)
- superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
- GDP-N-acetyl-α-D-perosamine biosynthesis (1/3 steps found)
- GDP-N-formyl-α-D-perosamine biosynthesis (1/3 steps found)
- GDP-mycosamine biosynthesis (1/3 steps found)
- superpathway of candicidin biosynthesis (4/11 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.2.1.47
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A3A4CGX4 at UniProt or InterPro
Protein Sequence (372 amino acids)
>DZA65_RS19730 GDP-mannose 4,6-dehydratase (Dickeya dianthicola ME23) MKKVALISGVTGQDGSYLAEFLLEKGYEVHGIKRRSSLFNTQRVDHLYQDPHEENVKFNL HYGDLTDTSNLTRIIAEVQPDEIYNLGAQSHVAVSFESPEYTADVDAIGTLRLLESIRFL GLADKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKMYAYWITVNYRESYGMYAC NGILFNHESPRRGETFVTRKITRGLSNIAQGLEKCIYMGNLDSLRDWGHARDYVRMQWMM LQQEKPEDFVIATGVQYSVRDFIRMSAVKLGIELEFTGTGVNEKAVVSKITGNNAPALKV GDVIVAIDPRYFRPAEVETLLGDPSKAKNKLGWVPETTLEEMIDEMVAYDLEKAKQFALL KSNGYDVSVSVE