Protein Info for DZA65_RS19445 in Dickeya dianthicola ME23

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 TIGR02350: chaperone protein DnaK" amino acids 3 to 603 (601 residues), 965.3 bits, see alignment E=5.3e-295 PF00012: HSP70" amino acids 4 to 602 (599 residues), 889.9 bits, see alignment E=6.9e-272 PF06723: MreB_Mbl" amino acids 114 to 376 (263 residues), 43.5 bits, see alignment E=2e-15

Best Hits

Swiss-Prot: 93% identical to DNAK_PECCP: Chaperone protein DnaK (dnaK) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 99% identity to ddc:Dd586_3594)

MetaCyc: 90% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4C8N4 at UniProt or InterPro

Protein Sequence (636 amino acids)

>DZA65_RS19445 molecular chaperone DnaK (Dickeya dianthicola ME23)
MGKIIGIDLGTTNSCVAIMDGTQVKVLENSEGDRTTPSIIAYTQDGETLVGQPAKRQAVT
NPKNTLFAIKRLIGRRFQDEEVQRDSNIMPYKIIPADNGDAWLEVKDQKLAPPQISAEVL
KKMKKTAEDYLGETITEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYG
LDKGIGNRTIAVYDLGGGTFDISIIEIDDVDGEKTFEVLATNGDTHLGGEDFDSRMINYL
VDEFKKEQGFDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHLNIKV
TRAKLESLVEDLVTRSLEPLKVALQDAGLSVSEIQDVILVGGQTRMPLVQKKVADFFGKE
PRKDVNPDEAVAIGAAVQGGVLAGDVKDVLLLDVTPLSLGIETMGGVMTPLITKNTTIPT
KHSQVFSTAEDNQSAVTIHVLQGERKRAHDNKSLGQFNLDGIQPSPRGMPQIEVTFDIDA
DGILHVSAKDKNSGREQNITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQARNQGD
HLLHSTRKQLEETGDKLAAADKTAIEEALKALETALKGEDKADIEAKIQQLAEASTKLLE
AAQQQQAQAGAAGADNAARQDDDVVDAEFEEVKDKK