Protein Info for DZA65_RS19415 in Dickeya dianthicola ME23

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 941 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 847 (833 residues), 962.8 bits, see alignment E=8.9e-294 PF00133: tRNA-synt_1" amino acids 28 to 644 (617 residues), 513.8 bits, see alignment E=7.8e-158 PF09334: tRNA-synt_1g" amino acids 58 to 201 (144 residues), 39.2 bits, see alignment E=9.3e-14 PF08264: Anticodon_1" amino acids 689 to 844 (156 residues), 103.4 bits, see alignment E=2.7e-33 PF06827: zf-FPG_IleRS" amino acids 901 to 929 (29 residues), 42.3 bits, see alignment (E = 1.3e-14)

Best Hits

Swiss-Prot: 81% identical to SYI_CROS8: Isoleucine--tRNA ligase (ileS) from Cronobacter sakazakii (strain ATCC BAA-894)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 79% identity to cko:CKO_03366)

MetaCyc: 80% identical to isoleucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Isoleucine--tRNA ligase. [EC: 6.1.1.5]; 6.1.1.5 [EC: 6.1.1.5]; 3.1.1.- [EC: 6.1.1.5]; RXN-23924 [EC: 6.1.1.5]

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y1W2 at UniProt or InterPro

Protein Sequence (941 amino acids)

>DZA65_RS19415 isoleucine--tRNA ligase (Dickeya dianthicola ME23)
MSDYKTTLNLPETGFPMRGDLAKREPDMLKRWYEQDLYGIIRGAKKGKKTFILHDGPPYA
NGNIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPIELKVEQLVGKPGEKVSAA
QFREECRKYAAEQVAGQKKDFIRLGVLGEWDRPYLTMDFKTEANIIRALGRIIENGHLHK
GAKPVHWCADCGSALAEAEVEYYDKTSPAIDVAFNAVDRAAVLAKFGLAADAVDGEVALV
IWTTTPWTLPANRAISLNAEFDYVLIQVAGKAVIVAEGLADAVAKRVTGSDAVTRLGHAV
KGSELELLRFRHPFMDFDVPAILGDHVTLDAGTGAVHTAGGHGPDDYVISQKYQLEIANP
VGPNGCYLPGTLPGLDGLFVFKANDKIVELLRERGALLHHEKLQHSYPCCWRHKSPILFR
ATPQWFVSMDQNGLRKQSLSEIKGVQWIPDWGQARIESMVANRPDWCISRQRTWGVPMSL
FVHKETQALHPRTAELIDAVAKHVEQDGIQAWWDLNPADLLGADAADYEKVPDTLDVWFD
SGSTHASVVDVRPEFSGHEADMYLEGSDQHRGWFMSSLMISTAIKGKAPYRQVLTHGFTV
DGQGRKMSKSIGNTVSPQDVMDKLGADILRLWIGSTDYSGEIAVSDEILKRSADAYRRIR
NTARFLLANLNGFDPAQHSVKPEDMVVLDRWAVSCAKAAQDEIVEAYESYDFHRVVQRLM
QFCSIEMGSFYLDIIKDRQYTAKHDSVARRSCQTALFHIAEALVRWMAPILSFTADEIWA
YLPGERAQFVFTEEWYDGLFGLADSEPMNDAFWADMLNVRGEVNKVIEQARNDKRIGGSL
EAAVTLYADDTLFAALSSLQGELHFALLTSKARLDRDENAPTDAQQSELPGLKVVLSKAD
GEKCPRCWHYETDIGSNAAHPDVCGRCATNVSGNGEERKFV