Protein Info for DZA65_RS19395 in Dickeya dianthicola ME23

Annotation: ribonucleoside hydrolase RihC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01156: IU_nuc_hydro
  • amino acids 6 to 294 (289 residues), 318.9 bits, E=2.2e-99

Best Hits

Swiss-Prot: 77% identical to RIHC_SALSV: Non-specific ribonucleoside hydrolase RihC (rihC) from Salmonella schwarzengrund (strain CVM19633)

KEGG orthology group: K12700, non-specific riboncleoside hydrolase [EC: 3.2.-.-] (inferred from 94% identity to ddd:Dda3937_01392)

MetaCyc: 74% identical to ribonucleoside hydrolase RihC (Escherichia coli K-12 substr. MG1655)
Adenosine nucleosidase. [EC: 3.2.2.7]; Uridine nucleosidase. [EC: 3.2.2.7, 3.2.2.3]; 3.2.2.3 [EC: 3.2.2.7, 3.2.2.3]; Inosine nucleosidase. [EC: 3.2.2.7, 3.2.2.3, 3.2.2.2]; Purine nucleosidase. [EC: 3.2.2.7, 3.2.2.3, 3.2.2.2, 3.2.2.1]

Predicted SEED Role

"Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)" in subsystem Purine conversions or Queuosine-Archaeosine Biosynthesis (EC 3.2.2.1)

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides salvage (10/10 steps found)
• adenine and adenosine salvage II (2/2 steps found)
• guanine and guanosine salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage III (2/2 steps found)
• guanosine nucleotides degradation II (3/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (4/6 steps found)
• purine nucleotides degradation I (plants) (8/12 steps found)
• adenosine nucleotides degradation I (5/8 steps found)
• guanosine nucleotides degradation I (2/4 steps found)
• superpathway of purines degradation in plants (11/18 steps found)
• NAD salvage (plants) (5/11 steps found)

KEGG Metabolic Maps

• Glycosaminoglycan degradation
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.1

Use Curated BLAST to search for 3.2.-.- or 3.2.2.1 or 3.2.2.2 or 3.2.2.3 or 3.2.2.7

Sequence Analysis Tools

See A0A385Y1U9 at UniProt or InterPro

Protein Sequence (305 amino acids)

>DZA65_RS19395 ribonucleoside hydrolase RihC (Dickeya dianthicola ME23)
MSRIPIILDTDPGIDDAVAIAAALFAPELELKLITTVAGNVDVEKTTRNALQLLHFWGST
VPVARGAATPLARVLRDAAYVHGESGMAGYDFVAHDRVALKKPAVQAMYECLTSSPEPMT
LVTIGPLTNIALLLTQYPQCKNNIKRLVMMGGSSGRGNFTPNAEFNIAIDPEAAGRVFDS
GIDIVMCGLDVTNNAVLAPDYLETLAGLNRTGAMLHALFSHYRSGSMATGLRMHDLCAIA
YLVKPALFTLTHCFVAVETRGEYTAGTTVVDLQHRLNRPANAQVALALDVDGFRAWVAQV
LALAP