Protein Info for DZA65_RS18490 in Dickeya dianthicola ME23

Annotation: family 1 glycosylhydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 PF00232: Glyco_hydro_1" amino acids 12 to 492 (481 residues), 426.5 bits, see alignment E=5e-132

Best Hits

Swiss-Prot: 62% identical to BGLH_BACSU: Aryl-phospho-beta-D-glucosidase BglH (bglH) from Bacillus subtilis (strain 168)

KEGG orthology group: K01223, 6-phospho-beta-glucosidase [EC: 3.2.1.86] (inferred from 96% identity to ddd:Dda3937_02590)

MetaCyc: 55% identical to 6-phospho-beta-glucosidase AscB (Escherichia coli K-12 substr. MG1655)
3.2.1.M25 [EC: 3.2.1.M25]; 6-phospho-beta-glucosidase. [EC: 3.2.1.M25, 3.2.1.86]

Predicted SEED Role

"6-phospho-beta-glucosidase (EC 3.2.1.86)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.86)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.86

Use Curated BLAST to search for 3.2.1.86 or 3.2.1.M25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y1D3 at UniProt or InterPro

Protein Sequence (496 amino acids)

>DZA65_RS18490 family 1 glycosylhydrolase (Dickeya dianthicola ME23)
MSVSTTCPPDVNAFPPDFLWGGATAANQLEGAWNVGGKGLSVSDVYTFDSRTPKARWLDQ
WLFMTHQQVAEAQDPHSQKYYPKRQGNGFYYHYKEDIALFAEMGFKCFRMSIAWTRIFPR
GDETEPNEDGLAFYDRVLDTLLAHNIQPIVSLSHYEMPLCLVTEYGGWPNRKLVDFYVRF
ATTVFERYRHKVKYWMTFNEINCVKHHPYVSVGVIEQDNPHLEQDKYQGVHHQFIASALA
TKACHQIIPGSQVGCMISYQMLYPHTCHPDDLQACEEAQRVSLFFSDVQARGYYPAYTDR
MLAEKGVVLKKAQGDDEMLRQYPVDYVSFSYYMSSTVSAHPEQLEGATGNLITGGIRNPY
LPTSEWGWQIDPKGLRLALNQLYDRYQKPLFIAENGLGAVDSVNPDGAIDDDYRIEYLRR
HVEQMKEALADGVKLFGYTWWGPIDVVSAGTAQISKRYGFIYVDQDDDGHGTQQRSRKKS
FYWYRKLIASNGADLD