Protein Info for DZA65_RS18240 in Dickeya dianthicola ME23

Annotation: bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 949 PF03710: GlnE" amino acids 38 to 279 (242 residues), 315.2 bits, see alignment E=3.2e-98 amino acids 559 to 811 (253 residues), 347 bits, see alignment E=6e-108 PF08335: GlnD_UR_UTase" amino acids 303 to 442 (140 residues), 109.1 bits, see alignment E=1.9e-35 amino acids 832 to 922 (91 residues), 38.6 bits, see alignment E=1.1e-13

Best Hits

Swiss-Prot: 77% identical to GLNE_PECAS: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 92% identity to ddc:Dd586_3344)

MetaCyc: 72% identical to fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase (Escherichia coli K-12 substr. MG1655)
GSDEADENYLATION-RXN [EC: 2.7.7.89]; [Glutamate--ammonia-ligase] adenylyltransferase. [EC: 2.7.7.89, 2.7.7.42]

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42 or 2.7.7.89

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4D149 at UniProt or InterPro

Protein Sequence (949 amino acids)

>DZA65_RS18240 bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase (Dickeya dianthicola ME23)
MTCHSASLFPLSELLTEQARHEAERLSISEPTPLADEAMAVLICSEFVSDALARYPQWRQ
EMVRQPPQPEEWRQYADWLSQALSGISDEAALMQALRLFRRRILTRIAWGQWLQLSTTGQ
TLQQLSVLAEVLIVAARQWLYDACCREWGTPCNAAGEPQPLLILGMGKLGGGELNFSSDI
DLIFAYPENGQTRGGRRELDNAQFFTRLGQRLIKALDQPTVDGFVYRVDMRLRPFGDSGP
LVMSYAALEDYYQEQGRDWERYAMVKARLMGGDDDHYSQELIRMLRPFVFRRYIDFSVIQ
SLRNMKNMIAREVRRRDLRNNIKLGAGGIREVEFITQVFQLIRGGREPALQGRALLPTLM
QVGELGLLTPEQAAQLREAYLFLRRLENLLQSIADEQTQTLPEDPLNQARLAWGMRCDDW
LQLSERLHQQMSAVRAVFHELIGDDAPDGSETPEHSHYSSLWQDGLDSADLAALAPELTP
EVCERLLARVTDFRADLSRRTIGPRGRDVLDQLMPALLAQACSHANADVIVARLTPLLLG
IVTRTTYLELLLESRSALTQLIWLCAVSPMIASQLARYPLLLDELLDPASLYQPTEQNAY
ADELRQYLMRVPEEDEEQQLEALRQFKQAQHLRIAAADIVGALPVMKVSDHLTYLAEAII
AAVVQQAWNQMAARYGQPAHLHSHSGRGFAVVGYGKLGGWELGYSSDLDLVFLIDCPDNV
MTDGERSIDGRQFYLRLAQRVMHLFSTRTSSGILYEVDARLRPSGAAGMLVSSVAAFEDY
QCNEAWTWEHQALVRARVVYGEPAIQQQFEQIRQRILCRERDGDALRTDVREMREKMRQH
HASKDSDFDIKADAGGITDIEFIAQYLVLRYAAQEPRLTRWSDNVRILELLARHGVMAEE
EADALRLAYITLRDEIHHLALQELPGRVDQDGFCAERQQVQQSWKKWLE