Protein Info for DZA65_RS18235 in Dickeya dianthicola ME23

Annotation: bifunctional D-glycero-beta-D-manno-heptose-7-phosphate kinase/D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase HldE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 475 TIGR02198: bifunctional protein RfaE, domain I" amino acids 6 to 312 (307 residues), 425.6 bits, see alignment E=1.7e-131 PF00294: PfkB" amino acids 12 to 305 (294 residues), 181.1 bits, see alignment E=5.2e-57 PF08543: Phos_pyr_kin" amino acids 189 to 287 (99 residues), 30.7 bits, see alignment E=3.1e-11 TIGR02199: bifunctional protein RfaE, domain II" amino acids 333 to 472 (140 residues), 209.1 bits, see alignment E=3.5e-66 TIGR00125: cytidyltransferase-like domain" amino acids 342 to 408 (67 residues), 63.1 bits, see alignment E=3.1e-21 PF01467: CTP_transf_like" amino acids 344 to 469 (126 residues), 70.5 bits, see alignment E=2.6e-23

Best Hits

Swiss-Prot: 91% identical to HLDE_PECCP: Bifunctional protein HldE (hldE) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K03272, D-beta-D-heptose 7-phosphate kinase / D-beta-D-heptose 1-phosphate adenosyltransferase [EC: 2.7.1.- 2.7.7.-] (inferred from 99% identity to ddd:Dda3937_00178)

MetaCyc: 86% identical to fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-4342 [EC: 2.7.7.70]; RXN0-4341 [EC: 2.7.7.70, 2.7.1.167]

Predicted SEED Role

"ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase" in subsystem LOS core oligosaccharide biosynthesis (EC 2.7.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.-, 2.7.7.-

Use Curated BLAST to search for 2.7.-.- or 2.7.1.- or 2.7.1.167 or 2.7.7.- or 2.7.7.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CLK0 at UniProt or InterPro

Protein Sequence (475 amino acids)

>DZA65_RS18235 bifunctional D-glycero-beta-D-manno-heptose-7-phosphate kinase/D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase HldE (Dickeya dianthicola ME23)
MKVTLPDFRHASVLVVGDVMLDRYWYGPTSRISPEAPVPVVKVDTIEERPGGAANVAMNI
AALGAGSRLVGLTGIDDAARALSAKLNEVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLD
FEEGFDSVDPQPMIERIQQALPKIGALVLSDYAKGALVHVQSMIQTARAAGVPVLIDPKG
TDFNRYRGATLLTPNLSEFEAVAGRCKDEHDLVERGAALLADLDLSALLVTRSEQGMTLL
QPGKAPLHLPTQAQEVYDVTGAGDTVIGVLAAALAAGKSLEEACFLANAAAGVVVGKLGT
STVTPIELENAIRGRADTGFGVMTEAQLKDAVALARQRGETVVMTNGCFDILHAGHVSYL
ANARRLGDRLIVAVNSDDSTKRLKGPSRPVNPLTQRMIVLGALEAVDWVVPFEEDTPQRL
ISEVLPDILVKGGDYKPEDIAGSKDVWANGGEVRVLNFEDGCSTTNIINTIKARD