Protein Info for DZA65_RS17235 in Dickeya dianthicola ME23

Annotation: class 1b ribonucleoside-diphosphate reductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 715 TIGR04170: ribonucleoside-diphosphate reductase, class 1b, alpha subunit" amino acids 16 to 715 (700 residues), 1175.5 bits, see alignment E=0 PF08343: RNR_N" amino acids 17 to 97 (81 residues), 100.7 bits, see alignment E=5.6e-33 PF00317: Ribonuc_red_lgN" amino acids 100 to 172 (73 residues), 63.6 bits, see alignment E=2.1e-21 TIGR02506: ribonucleoside-diphosphate reductase, alpha subunit" amino acids 104 to 698 (595 residues), 520.9 bits, see alignment E=4.6e-160 PF02867: Ribonuc_red_lgC" amino acids 177 to 695 (519 residues), 514.7 bits, see alignment E=4.3e-158

Best Hits

Swiss-Prot: 75% identical to RIR3_ECOLI: Ribonucleoside-diphosphate reductase 2 subunit alpha (nrdE) from Escherichia coli (strain K12)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 96% identity to ddd:Dda3937_03977)

MetaCyc: 75% identical to ribonucleoside-diphosphate reductase 2 subunit alpha (Escherichia coli K-12 substr. MG1655)
Ribonucleoside-diphosphate reductase. [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]

Predicted SEED Role

"Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4C8N9 at UniProt or InterPro

Protein Sequence (715 amino acids)

>DZA65_RS17235 class 1b ribonucleoside-diphosphate reductase subunit alpha (Dickeya dianthicola ME23)
MATAEFLTEAPSSQLDYHALNAMLNLYDADGRIQFEQDWQAIQAYFHNHVLPRTRRFPSL
EEKLRWLQQEGYYEPQVLAHYQPDFIAELFQRAAKHRYQFQSFLGAFKFYTSYALKTFDG
SQYLESYDDRACMVSLTLAQGDTQLAQQLVDEMMSGRFQPATPTFLNCGKQQRGELVSCF
LLRIEDNMESIGRAVNSALQLSKRGGGVAFMLTNIRETGAPIKRIENQSSGVIPIMKMLE
DAFSYANQLGARQGAGAVYLHAHHPDILRFLDTRRENADEKIRIKTLSLGVVIPDITLRL
ARANQDMHLFSPYDVERVYGVPLSEISVSEKYDELVSHPEIRNWTINARQFFQTLAEIQF
ESGYPYLMFEDTVNRHNPVAGRITMSNLCSEILQVSRASEYQEDLNYRQVGKDISCNLGS
LNIARVMDGGNLAHSVEIAVRALTAVSDMSHIGAVPSIANGNAESHAIGLGQMNLHGYLA
REGLFYGSEEALDFTNLYFYAVTYHALRTSSLLAQERNQTFAGFVDSQYASGAFFERYLS
QEWQPATKRGRELFASAGIALPLREDWLALKAQVMRHGLYNQNLQAVPPTGSISYINHAT
ASIHPIVSRIEIRKEGKLGRVYYPAPYMTNDNQRFYQDAYEIGPEKIIDTYAVATRHVDQ
GLSLTLFFPDTATTRDINKAQIYAWKKGIKTLYYIRVRQKALEGTEIQGCVSCSL