Protein Info for DZA65_RS17115 in Dickeya dianthicola ME23

Annotation: bifunctional glycosyl transferase/transpeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 832 transmembrane" amino acids 66 to 87 (22 residues), see Phobius details PF14812: PBP1_TM" amino acids 1 to 80 (80 residues), 100.9 bits, see alignment E=7.7e-33 TIGR02071: penicillin-binding protein 1B" amino acids 69 to 795 (727 residues), 1215.3 bits, see alignment E=0 PF14814: UB2H" amino acids 114 to 198 (85 residues), 91.9 bits, see alignment E=4.2e-30 PF00912: Transgly" amino acids 210 to 381 (172 residues), 192.5 bits, see alignment E=9.1e-61 PF00905: Transpeptidase" amino acids 474 to 713 (240 residues), 87.2 bits, see alignment E=2.4e-28

Best Hits

Swiss-Prot: 70% identical to PBPB_ECOLI: Penicillin-binding protein 1B (mrcB) from Escherichia coli (strain K12)

KEGG orthology group: K05365, penicillin-binding protein 1B [EC: 2.4.1.129 3.4.-.-] (inferred from 94% identity to dze:Dd1591_1036)

MetaCyc: 70% identical to peptidoglycan glycosyltransferase / peptidoglycan DD-transpeptidase MrcB (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]; 3.4.16.4 [EC: 3.4.16.4]; Peptidoglycan glycosyltransferase. [EC: 3.4.16.4, 2.4.1.129]

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-, 3.4.16.4

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.- or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y4V9 at UniProt or InterPro

Protein Sequence (832 amino acids)

>DZA65_RS17115 bifunctional glycosyl transferase/transpeptidase (Dickeya dianthicola ME23)
MSRDDREPIGRKRSAPRHTPPRRQATRRRRDDDYDDDYEEEYEEDYDDEDDEEEVMSKRP
RKKRRWLGWLIRLFLIFVVVMSIYGVYLDGEIRSRIDGKVWQLPAAVYGRMVNLEPGMAY
SKKEMVNLLEGMQYREVSRITRPGEFTVQANGIEILRRPFDFPDGKEGQIHAKMIFSNDR
LSQIQNMDNQRNFGFFRLDPKLITMLQSPNGEQRLFVQRSGFPDLLVQTLIATEDRHFYE
HDGISFYSIGRALIANLTAGRAVQGGSTLTQQLVKNLFLTNERSLWRKANEAYMALIMDY
RYSKDRILELYLNEVYLGQSGNDQIRGFPLASLYYFGRPVNELSLDQQAMLVGMVKGASL
YNPWRNPQVTLERRNLVLKLLQNQQVIDEDLYNMLSARPLGVQPKGGVISPQPAFMQMVR
QELQQRLGDKVNDLSGVKIFTTLDPVSQDAAEKAVEDGIPALRAARGVKDLESAMVIVDR
FSGEIRAMVGGSDAQFAGFNRAMQARRSVGSLAKPPTYLTALSMPDRYRLNTILADEPIS
LKQPNGTVWEPKNYDREFRGRVMLVDALTNSLNVPTVNLGMAVGLDQVSDTLQKLGIPQN
QIQAVPAMLLGAVSLTPMEVAQEYQTIASGGNRASLSALRSVIAEDGKVLYQSFPQAERA
VPSQAAYLTLYGMQQVVERGTSRSLTVKFGNYHLAGKTGTTNDLRDSWFAGVDGKEVAIT
WVGRDNNGPAKLTGANGALTIYRRYLENQTPLPLMLTPPEGITTMSVDGSGNVICNGSAG
RALPVWTDNPQALCQASQPAVQQPANAPNGQPQQNQQEGGSVPDWIKQMFGQ