Protein Info for DZA65_RS17095 in Dickeya dianthicola ME23

Annotation: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01704: MTA/SAH nucleosidase
  • amino acids 2 to 228 (227 residues), 375.6 bits, E=4.2e-117
• PF01048: PNP_UDP_1
  • amino acids 2 to 226 (225 residues), 192.9 bits, E=2.9e-61

Best Hits

Swiss-Prot: 86% identical to MTNN_PECAS: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K01243, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase [EC: 3.2.2.9] (inferred from 97% identity to ddd:Dda3937_00498)

MetaCyc: 76% identical to adenosylhomocysteine nucleosidase (Escherichia coli ATCC 25922)
Methylthioadenosine nucleosidase. [EC: 3.2.2.16, 3.2.2.9]; 3.2.2.9 [EC: 3.2.2.16, 3.2.2.9]; 3.2.2.9 [EC: 3.2.2.16, 3.2.2.9]

Predicted SEED Role

"5'-methylthioadenosine nucleosidase (EC 3.2.2.16) / S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)" in subsystem Adenosyl nucleosidases or Methionine Salvage or Polyamine Metabolism or Methionine Biosynthesis or Methionine Degradation (EC 3.2.2.16, EC 3.2.2.9)

MetaCyc Pathways

• L-methionine salvage cycle I (bacteria and plants) (11/12 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• L-methionine salvage cycle II (plants) (9/11 steps found)
• autoinducer AI-2 biosynthesis II (Vibrio) (5/6 steps found)
• S-methyl-5'-thioadenosine degradation I (2/2 steps found)
• autoinducer AI-2 biosynthesis I (4/5 steps found)
• S-methyl-5'-thioadenosine degradation IV (1/1 steps found)
• 5'-deoxyadenosine degradation II (3/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (4/7 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.16 or 3.2.2.9

Sequence Analysis Tools

See A0A3A4CBY7 at UniProt or InterPro

Protein Sequence (233 amino acids)

>DZA65_RS17095 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (Dickeya dianthicola ME23)
MKVGIIGAMEQEVALLREQIQNRQTFQRAGCEIYSGQLHGVEIALLKSGIGKVSAALGTT
LLLEHGQPDVVINTGSAGGLASSLKVGDIVVSDEVRYHDADVTAFGYEPGQMAGCPAAFT
ADEKLIALAQDAIGQLQLNAVRGLVVSGDAFINGKAPLARIRQTFPQAIAVEMEATAIGH
VCHQFGVPFVVVRAISDVADQESHLSFDEFLVTAAKQSTRMVETMLQSLAQHR