Protein Info for DZA65_RS16830 in Dickeya dianthicola ME23

Annotation: phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1294 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 3 to 1292 (1290 residues), 2036.5 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 149 (115 residues), 152 bits, see alignment E=1.5e-48 PF18072: FGAR-AT_linker" amino acids 170 to 219 (50 residues), 80.1 bits, see alignment (E = 3.5e-26) PF02769: AIRS_C" amino acids 431 to 586 (156 residues), 114.6 bits, see alignment E=1.2e-36 amino acids 830 to 965 (136 residues), 49.2 bits, see alignment E=1.7e-16 PF22689: FGAR-AT_PurM_N-like" amino acids 646 to 805 (160 residues), 279.7 bits, see alignment E=1.6e-87 PF13507: GATase_5" amino acids 1038 to 1291 (254 residues), 365 bits, see alignment E=4.3e-113

Best Hits

Swiss-Prot: 82% identical to PUR4_ECO57: Phosphoribosylformylglycinamidine synthase (purL) from Escherichia coli O157:H7

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 71% identity to aap:NT05HA_1480)

MetaCyc: 81% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y0E0 at UniProt or InterPro

Protein Sequence (1294 amino acids)

>DZA65_RS16830 phosphoribosylformylglycinamidine synthase (Dickeya dianthicola ME23)
MEILRGSPALSAFRINKLLARCKEYYLPVSDIYAEYVHFADVNAPLNHEEQSRLSRLLKY
GPSLAEHEPTGRLILVTPRPGTISPWSSKATDIAHNCGLQKIRRLERGLAFYIHAPTLSD
AQWRELAALLHDRMMESVFDDLQQANLLFSQHQPASLKRVEILLQGRPALEDANLRLGLA
LADDEMDYLLDAFTKLGRNPTDIELYMFAQANSEHCRHKIFNADWIINGEAQPKSLFKMI
KNTFEHTPDYVLSAYKDNAAVMEGSAVGRFFPDPQGAYGYHQEDAHILMKVETHNHPTAI
SPWPGAATGSGGEIRDEGATGRGAKPKAGLVGFSVSNLRIPGFIQPWEQDFGKPDRIVSA
LDIMTDGPLGGAAFNNEFGRPALTGYFRTYEEVVDSHNGVEVRGYHKPVMLAGGIGNICA
GHVKKGEISIGAKLIVLGGPAMNIGLGGGAASSMTSGQSDADLDFASVQRDNPEMERRCQ
EVIDRCWQLGEQNPILFIHDVGAGGLSNAMPELVSDGGRGGRFELRDILNDEPGMSPLEV
WCNESQERYVLAVAPEQLALFDDICRRERAPYAVIGEATEEQHLTLNDRHFNNQPIDMPL
DVLLGKTPKMLRNVERKDVEGTPLNREGIYLAQAVERVLHLPAVAEKTFLITIGDRTVTG
MVARDQMVGPWQVPVADCAVTTASLDSYYGEAMSIGERAPVALRNFAASARLAVGEALTN
IAATHIGDLKRVKLSANWMAAAGHPGEDAGLYDAVKAVGEELCPALGLTIPVGKDSMSMK
TRWQENGEDKSVTAPLSLVISAFARVEDVRHTVTPQLRTDKDNVLLMIDLGAGHHALGAT
ALAQVYRQLGRKTADVRNPVQLAGFFNAMQALVANQALLAYHDRSDGGLLVTLAEMAFAG
HCGINADIGSMGEDALAVLFNEELGAVIQIDAARRAEVEQVLAEHGLADCVHYLGQAEAG
NQFIIHCGNDVVYHESRTTLRNWWAETTWQMQRLRDNPQCADQEHHTKSDDNDPGLNVTL
TFDLREDIAAPFISRQARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDIHMSDLLAGRRN
LQDFQALVACGGFSYGDVLGAGEGWAKSILFNDRVRDEFAAFFLRPQTLALGVCNGCQMM
SNLRELIPGAEHWPRFVRNKSDRFEARFSLVEVTHSPSLFLQDMAGSRMPIAVSHGEGRL
EVRDDSHLTALEQHQLVALRYVNNYGQATEDYPANPNGSPNGITAVTSASGRATVMMPHP
ERVFRTVSNSWHPEAWGEDSPWMRMFRNARRQLG