Protein Info for DZA65_RS16790 in Dickeya dianthicola ME23

Annotation: polysaccharide lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 signal peptide" amino acids 1 to 42 (42 residues), see Phobius details PF00544: Pectate_lyase_4" amino acids 95 to 324 (230 residues), 313.1 bits, see alignment E=4.7e-98

Best Hits

Swiss-Prot: 95% identical to PLYE2_DICCH: Pectate lyase E (pelE) from Dickeya chrysanthemi

KEGG orthology group: K01728, pectate lyase [EC: 4.2.2.2] (inferred from 95% identity to ddd:Dda3937_03371)

MetaCyc: 95% identical to pectate lyase E (Dickeya dadantii 3937)
Pectate lyase. [EC: 4.2.2.2]

Predicted SEED Role

"FIG00613867: hypothetical protein"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.2.2

Use Curated BLAST to search for 4.2.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y3U8 at UniProt or InterPro

Protein Sequence (404 amino acids)

>DZA65_RS16790 polysaccharide lyase (Dickeya dianthicola ME23)
MNNSRMSSVSTQKTTGRSTLGTQSALAAIIATTMMVSAASAASLQTTKATEAASTGWATQ
TTGTTGGAKATSAKIYAVKSISEFKTALNGTDSSPKIIQITGTIDISGGKAYTSFDDQKA
RSQISIPSNTTIIGIGSNGKFTNGSLVVKGVSNVILRNLYIETPVDVAPHYEDGDGWNAE
WDAVVIDSTDHVWVDHVTISDGSFTDDKYTTKDGEKYVQHDGALDIKRGSDYVTISNSRF
ELHDKTILIGHSDNNGSQDTGKLRVTFHNNLFDRVGERTPRVRFGSVHAYNNVYVGDVNH
KAYRYQYSFGIGTSGSLLSESNAFTIDNLKKISGRDKECSVVKAFNGKIFSDKGSIINGA
SYNLNGCDFGFSAYSAKIPYKYSAQTITTSLASSISNNAGYGKL