Protein Info for DZA65_RS16120 in Dickeya dianthicola ME23

Annotation: DNA helicase IV

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 685 PF12462: Helicase_IV_N" amino acids 1 to 164 (164 residues), 233.4 bits, see alignment E=3.6e-73 PF00580: UvrD-helicase" amino acids 197 to 490 (294 residues), 161 bits, see alignment E=1.2e-50 PF13245: AAA_19" amino acids 207 to 475 (269 residues), 76.2 bits, see alignment E=7.2e-25 PF13361: UvrD_C" amino acids 562 to 661 (100 residues), 40.7 bits, see alignment E=5.4e-14 PF13538: UvrD_C_2" amino acids 629 to 660 (32 residues), 29.8 bits, see alignment (E = 1.1e-10)

Best Hits

Swiss-Prot: 66% identical to HELD_ECOLI: DNA helicase IV (helD) from Escherichia coli (strain K12)

KEGG orthology group: K03658, DNA helicase IV [EC: 3.6.4.12] (inferred from 96% identity to ddd:Dda3937_02829)

MetaCyc: 66% identical to DNA helicase IV (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"DNA helicase IV" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y017 at UniProt or InterPro

Protein Sequence (685 amino acids)

>DZA65_RS16120 DNA helicase IV (Dickeya dianthicola ME23)
MELKSTALGKHLAQHPYNRVQLLNAGVNVSGDRHEYLIPFNQLLAIRCKKGLIWGELEFE
LPENKVVRLHGTEWQETQQFFRHLFKSWQSWSQEMSEVCKDVLQQLVDTIADRETADGWF
SRQALAHVQQDIRAGFAALPLPLARLDEFDHCRDNYQRCLRWLEQGEALRQQANQRWTET
ILVEQNAFFDTVESSPLNPSQCQAVVNGENAVLVLAGAGSGKTSVLVARAAWLLHRAEAS
AQQILLLAFGRQAADEMNSRIRERLHTDEVQAKTFHALALHIIQQCSRKAPVISRLETDG
AHRRAFLIQHWQQQCDEKKTQAKGWRQWLTEELEWTLPEGNFWQDAAIASRLAGRLERWL
GLMRMQGGTQSEMLALADDETRPLFQQRLRLMAPLLKAWKKALKDEGAVDFSGLIHQAVN
FLDKGRFVSPWRHILVDEFQDISPQRARLLDALRRQIPDSSLFAVGDDWQAIYRFSGAEL
TLTTTFERHFGEGAQCVLDTIYRFNHRIGDIASRFIQQNPAQLKKALNSLRDGNKKSVVL
LAQEQLEALLDKMSGYVTTQARILVLARYHHLRPDALEKAQTRWPHLHLDFMTIHASKGQ
QADYVIVLGLHEGRDGFPAPPRESVLEAVLLPQPEPFPDAEERRLLYVALTRAKHQVWLL
YDRDEPSVFVDDLHQLGVPIQRKPG