Protein Info for DZA65_RS16050 in Dickeya dianthicola ME23

Annotation: exopolyphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 509 TIGR03706: exopolyphosphatase" amino acids 14 to 307 (294 residues), 328.5 bits, see alignment E=2e-102 PF02541: Ppx-GppA" amino acids 27 to 309 (283 residues), 333 bits, see alignment E=1.7e-103 PF21447: Ppx-GppA_III" amino acids 315 to 491 (177 residues), 200.2 bits, see alignment E=2.6e-63

Best Hits

Swiss-Prot: 74% identical to PPX_SALTY: Exopolyphosphatase (ppx) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 97% identity to ddd:Dda3937_01692)

MetaCyc: 72% identical to exopolyphosphatase (Escherichia coli K-12 substr. MG1655)
Exopolyphosphatase. [EC: 3.6.1.11]

Predicted SEED Role

"Exopolyphosphatase (EC 3.6.1.11)" in subsystem Phosphate metabolism (EC 3.6.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.11, 3.6.1.40

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CEQ0 at UniProt or InterPro

Protein Sequence (509 amino acids)

>DZA65_RS16050 exopolyphosphatase (Dickeya dianthicola ME23)
MPLTNNNTEQPQEFAAVDLGSNSFHMVVARVVNGALQVLSRLKQRVHLADGLDNQNQLSE
ESIQRGLSCLALFAERLQGFPATNVSIVGTHALRQAVNAQDFLRRAARIIPYPIEIISGH
EEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGEDFEPMLVESRRMGCVSFAQQFFPN
GEISEVNFRRARLAAAQKLETLAWEYRIYGWDYALGASGTIKATCEILVAMGEKDGLITP
ERLEMLRERILQFKNFRAVSLPGLTEDRQNVLVPGFAILCGVFDALAIKELRLSDGALRE
GVLYEMEGRFRHQDIRIRTAQSLASHYNIDREQAKRVRETADQLYAQWAQQNLSLVHPQL
EALLKWSAMLHEVGLGINHSGMHRHSAYILQNTNLPGFNQEQQQLLALMVRLHRKAVKLE
ELPRFNLFKKKQYLPMVQLLRLATLLNNQRQATTTPKTLRLIASETTWTLIFPQGFFNQN
MLVQLDLEREQQYWEDSSGWKLHIEEELV