Protein Info for DZA65_RS16030 in Dickeya dianthicola ME23

Annotation: phosphate ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 271 TIGR00972: phosphate ABC transporter, ATP-binding protein" amino acids 25 to 271 (247 residues), 385.5 bits, see alignment E=4.8e-120 PF00005: ABC_tran" amino acids 40 to 196 (157 residues), 111.4 bits, see alignment E=8.4e-36 PF13304: AAA_21" amino acids 126 to 225 (100 residues), 30.7 bits, see alignment E=4.9e-11

Best Hits

Swiss-Prot: 82% identical to PSTB1_PECAS: Phosphate import ATP-binding protein PstB 1 (pstB1) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K02036, phosphate transport system ATP-binding protein [EC: 3.6.3.27] (inferred from 98% identity to ddd:Dda3937_01688)

MetaCyc: 60% identical to phosphate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.27

Use Curated BLAST to search for 3.6.3.27 or 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CPX3 at UniProt or InterPro

Protein Sequence (271 amino acids)

>DZA65_RS16030 phosphate ABC transporter ATP-binding protein (Dickeya dianthicola ME23)
MGMAKQLERLPVMDVHRLTDDQTALSVDGLNLYYGDKQALSDISLRIPKHRVTALIGPSG
CGKSTLLRCFNRMNDLMDTCRLEGDIRLQGMRINDPSLDVSTLRRRVGMVFQRPNPFPKS
IYENVIYGLRLQGVKDRRMLDEAVENALRAAALWHEVKDRLGDNALTLSSGQQQRLVIAR
AIAIEPEVLLLDEPTSALDPISTLVIEELIGALKRRFTLVLVTHNMQQAARVSDYTAFIH
HGRLIEYNETDALFTAPVERRTEDYITGRFG