Protein Info for DZA65_RS15970 in Dickeya dianthicola ME23

Annotation: cysteine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 TIGR00435: cysteine--tRNA ligase" amino acids 2 to 460 (459 residues), 641.5 bits, see alignment E=5.1e-197 PF01406: tRNA-synt_1e" amino acids 15 to 313 (299 residues), 494.3 bits, see alignment E=3.6e-152 PF09334: tRNA-synt_1g" amino acids 36 to 106 (71 residues), 28.4 bits, see alignment E=1.8e-10 amino acids 249 to 310 (62 residues), 25.2 bits, see alignment E=1.7e-09 PF09190: DALR_2" amino acids 341 to 395 (55 residues), 82.3 bits, see alignment 7.5e-27 PF23493: CysS_C" amino acids 414 to 459 (46 residues), 38.9 bits, see alignment 2e-13

Best Hits

Swiss-Prot: 84% identical to SYC_PECCP: Cysteine--tRNA ligase (cysS) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 96% identity to ddd:Dda3937_01678)

MetaCyc: 82% identical to cysteine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4D7F9 at UniProt or InterPro

Protein Sequence (461 amino acids)

>DZA65_RS15970 cysteine--tRNA ligase (Dickeya dianthicola ME23)
MLKIFNTLSRQKEEFNPIHAGKVGMYVCGITVYDLCHIGHGRTFVAFDVVARYLRYLGYD
VKYVRNITDIDDKIIKRATENGETITQLTDRMIGDMHTDFNALNIQRPDEEPRATHYIAE
IIALVEKLLARGHAYVADNGDVMFSVDSAPGYGALSRQDLEQLKAGARVEITEVKRNPMD
FVLWKMSKAGEPSWPSPWGNGRPGWHIECSAMNCKQLGEHFDIHGGGSDLMFPHHENEIA
QSTCAHGGEYVNYWMHTGMVMVDREKMSKSLNNFFTMRDVLMHYDAETIRYFLISGHYRS
QLNYTEENLKQARASLERLYTALRGTDAAVPAAGGDAFESRFRDAMNDDFNTPEAYSVLF
DMAREINRLKAEDTAAANGLAAALRRLAGVLGLLERDPELFLQSGAQADDGEVQEIEALI
KQRNDARQSKDWALADAARNRLTEMGIVLEDGPQGTIWRRK