Protein Info for DZA65_RS15835 in Dickeya dianthicola ME23

Annotation: histidine ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01225: histidine ammonia-lyase
  • amino acids 10 to 503 (494 residues), 684 bits, E=5.5e-210
• PF00221: Lyase_aromatic
  • amino acids 15 to 472 (458 residues), 612.1 bits, E=3.1e-188

Best Hits

Swiss-Prot: 71% identical to HUTH_ACIAC: Histidine ammonia-lyase (hutH) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 95% identity to dze:Dd1591_1264)

MetaCyc: 62% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

See A0A3A4CPI0 at UniProt or InterPro

Protein Sequence (518 amino acids)

>DZA65_RS15835 histidine ammonia-lyase (Dickeya dianthicola ME23)
MSQTRSYCLTPGAVDLATLRAVYQGNVTLTLAAQARAAAAAAQETVQRIVRQDRVVYGIN
TGFGKLAQTRIPADRLAELQRNLVLSHSVGIGDLLADNVLRLVMATKVISLARGHSGVRL
QVIDTLLALFNAGVMPCIPEKGSVGASGDLAPLAHLSLLLLGEGQARVDGVLIPAADGLA
RAGVEPLVLGPKEGLALLNGTQVSTALALRGLFEAENVFAAGLVAGALSLEAVKGSVKPF
DARIHQARGQHGQMAVAAAVSSLLTDSEILQSHTHCGRVQDPYSIRCVPQVMGACLDNLQ
HAAHVLRIEANAASDNPLVFPETDEVISGGNFHAEPVAFAADIIALAVAEIGAISERRMA
LLLDSTLSGLPPFLVNDGGVNSGFMIAQVTAAALASENKSLAHPSSVDSLPTSANQEDHV
SMATYAARRLGSMCFNTAAVVGIEAMAAAQGIDFHRPLKSSSLLECELAQVRTQVAFLDH
DRLMATDIDLMRQWASRERWPAVITALLPSYAVNPASV