Protein Info for DZA65_RS14950 in Dickeya dianthicola ME23

Annotation: NADH-quinone oxidoreductase subunit NuoI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01971: NADH-quinone oxidoreductase, chain I
  • amino acids 16 to 137 (122 residues), 160.1 bits, E=8.3e-52
• PF13237: Fer4_10
  • amino acids 57 to 110 (54 residues), 30.5 bits, E=1.6e-10
• PF00037: Fer4
  • amino acids 57 to 76 (20 residues), 22.2 bits, see alignment (E = 5.1e-08)
  • amino acids 93 to 115 (23 residues), 34.4 bits, see alignment (E = 7.3e-12)
• PF12800: Fer4_4
  • amino acids 58 to 73 (16 residues), 16.5 bits, see alignment (E = 5e-06)
  • amino acids 97 to 111 (15 residues), 15 bits, see alignment (E = 1.4e-05)
• PF13187: Fer4_9
  • amino acids 59 to 114 (56 residues), 28.7 bits, E=5.8e-10
• PF12838: Fer4_7
  • amino acids 59 to 113 (55 residues), 44 bits, E=1.4e-14

Best Hits

Swiss-Prot: 93% identical to NUOI_PECAS: NADH-quinone oxidoreductase subunit I (nuoI) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00338, NADH dehydrogenase I subunit I [EC: 1.6.5.3] (inferred from 98% identity to dda:Dd703_2543)

MetaCyc: 91% identical to NADH:quinone oxidoreductase subunit I (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
• NAD(P)/NADPH interconversion (4/6 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• aerobic respiration I (cytochrome c) (2/4 steps found)
• Fe(II) oxidation (1/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

See A0A3A4CUJ5 at UniProt or InterPro

Protein Sequence (180 amino acids)

>DZA65_RS14950 NADH-quinone oxidoreductase subunit NuoI (Dickeya dianthicola ME23)
MTLKELLIGFGTQVRSICMVGSNAFKKRETKMYPEEPVNPPPRFRGRIVLTRDPDGQERC
VACNLCAVACPVGCISLQKAEMKDGRWYPEFFRINFSRCIFCGMCEEACPTTAIQLTPDF
EMGEFKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAVDGKEKGEAENEAKPIDVKGLLP