Protein Info for DZA65_RS14830 in Dickeya dianthicola ME23

Annotation: formate dehydrogenase-N subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1016 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 2 to 1015 (1014 residues), 1549.2 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 45 to 103 (59 residues), 53.8 bits, see alignment 2.3e-18 PF00384: Molybdopterin" amino acids 107 to 590 (484 residues), 104.9 bits, see alignment E=7.2e-34 PF01568: Molydop_binding" amino acids 902 to 1009 (108 residues), 44.6 bits, see alignment E=2e-15

Best Hits

Swiss-Prot: 72% identical to FDOG_ECOLI: Formate dehydrogenase-O major subunit (fdoG) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 97% identity to ddc:Dd586_2718)

MetaCyc: 72% identical to formate dehydrogenase O subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4D441 at UniProt or InterPro

Protein Sequence (1016 amino acids)

>DZA65_RS14830 formate dehydrogenase-N subunit alpha (Dickeya dianthicola ME23)
MQVSRRQFFKICAGGMAGTTVTALGFSPSAALAETRQYKLLRARETRNNCTYCSVGCGIL
MYSLGDGAKNASASIYHIEGDPDHPVSRGSLCPKGAGLVDFIHSDQRLKYPEYRAPGSDK
WQRISWEEAFDRITRLMKKDRDANFVEKNAAGVTVNRWLTTGMLCSSAASNETGILDQKF
ARALGMIGIDTQARLCHGPTVAALAPTFGRGAMTNNWVDIKNANVILVMGGNPAEAHPVG
FKWAVEAKIHNGAKLIVVDPRFNRSAAVADMYSPIRAGSDITFLLGVVNYLLSYDKVQMP
YVKAYTNASLIVRDDYHFDEGIFSGYDEQKRQYDRTSWQYELDENGFAKRDDTLSHPRCV
WNLLKEHASRYTPDLVTNVCGTPKPDFLTICEQLASTCVPDRTATIMYALGWTQHTAGAQ
IIRTAAMIQLLLGNIGMAGGGVNALRGHSNIQGYTDLGLLSLNLPGYMPLPSEKQTSLNM
YLDQITPKALLADQVNYWKNTPKFFISMMKSFWGNKATKENDWGYDWLPKWDKSYDALAY
SRMMLDGKVNGYIVQGFNPMAAFADSNRARDALKKLKYLVIIDPLATETSNFWQNHGELN
DVDPGKIQTEVFRLPSSCFAEENGSIVNSGRWLQWHYQGAEPPGEARHDGVILAGLFLRL
RELYAKEGGANPEPVLNMSWDYLEPEDPSAEEITKEANGRALADLYDDKGNLLLKKGQLL
PDFSLLRDDGSTASFCWIYAGSWTEAGNQMARRDNADPSGLGCTPGWAWCWPQNRRILYN
RASADEQGRPWDPKRELIHWNGQKWTGIDVPDFAATVPPGSAAGPFIMNAEGLGRLFAID
KMAEGPFPAHYEPLESPLKTNPVYSKVQVNPAARILPADRPYMGTVEEFPFVATTYSITE
LFRHWTKHARLNAIVQPEQFVEIGEGLAKLKGIQGGDVVKVSSKRGYIKAKAVVTKRIRT
LTIQGKPVETIGVPCHWGFEGTTQKGFMANILTPHVGDANSQTPEYKAFLVNVEKA