Protein Info for DZA65_RS14785 in Dickeya dianthicola ME23

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 381 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 35 to 363 (329 residues), 267.1 bits, see alignment E=9.1e-84 PF16576: HlyD_D23" amino acids 58 to 281 (224 residues), 51.6 bits, see alignment E=1.2e-17 PF13533: Biotin_lipoyl_2" amino acids 61 to 107 (47 residues), 37.2 bits, see alignment 2.9e-13 PF13437: HlyD_3" amino acids 171 to 278 (108 residues), 29.7 bits, see alignment E=1.3e-10

Best Hits

Swiss-Prot: 48% identical to ACRA_ECOLI: Multidrug efflux pump subunit AcrA (acrA) from Escherichia coli (strain K12)

KEGG orthology group: K03585, membrane fusion protein (inferred from 93% identity to ddd:Dda3937_01839)

MetaCyc: 48% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, membrane fusion protein CmeA" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CTF2 at UniProt or InterPro

Protein Sequence (381 amino acids)

>DZA65_RS14785 efflux RND transporter periplasmic adaptor subunit (Dickeya dianthicola ME23)
MRSKTFIVTAGMLTVLLTGCERNTSSTEAAQPVAVTVQTLHGAPVTLHSELTGRVTAAMV
SEVRPQVDGIIQQRLFTEGSEVQAGQVLYQIDPASYQATVDQAAAALKNAQSAVRSAKLK
AERYARLLKEEWVSQQDADDAQAAYEQDVASVAEKAAALKTAQINLAYTRITAPISGRIG
ISAVTPGALVTASQSTALATIRQLDPIYVDLTQSSSQRLALLAHQKQQQAAVTLTLENGQ
PYRQPGVLKLAEVAVDEATGSVTLRAEFPNAGHRLLPGMFVRATVETASVPDAILAPQQG
ILRDTKGNAYALVVNNQQVVERREVETGEAMGSRWLITQGLNAGDRLVTEGTDKVRAGDN
VTAVEESAGADSAMPRTTEAR