Protein Info for DZA65_RS14780 in Dickeya dianthicola ME23

Annotation: multidrug efflux RND transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1053 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 341 to 360 (20 residues), see Phobius details amino acids 367 to 389 (23 residues), see Phobius details amino acids 395 to 414 (20 residues), see Phobius details amino acids 442 to 461 (20 residues), see Phobius details amino acids 473 to 498 (26 residues), see Phobius details amino acids 540 to 558 (19 residues), see Phobius details amino acids 875 to 894 (20 residues), see Phobius details amino acids 901 to 921 (21 residues), see Phobius details amino acids 928 to 951 (24 residues), see Phobius details amino acids 976 to 996 (21 residues), see Phobius details amino acids 1004 to 1030 (27 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 2 to 1043 (1042 residues), 1530.2 bits, see alignment E=0 PF00873: ACR_tran" amino acids 2 to 1031 (1030 residues), 1221 bits, see alignment E=0 PF03176: MMPL" amino acids 304 to 497 (194 residues), 26.9 bits, see alignment E=3.5e-10

Best Hits

Swiss-Prot: 57% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 61% identity to abu:Abu_2161)

MetaCyc: 55% identical to multidrug efflux pump RND permease AcrD (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-360

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CTQ4 at UniProt or InterPro

Protein Sequence (1053 amino acids)

>DZA65_RS14780 multidrug efflux RND transporter permease subunit (Dickeya dianthicola ME23)
MFSRFFIHRPVFAWVIAIVIMLAGALSIESLPVAQYPNVAPPSIAIKAGYPGASADTLEN
SVTQVIEQQLTGLDGLLYFSSSSDSSGDVRITATFTQGTNPDTAQVQVQNKVQQATSRLP
TEVQQQGITVTKSQQDFLLIMALYDTTDKSTAADIADYMVSNLQDPLSRVEGVGSVQVFG
SQYAMRIWLDPIKLAAYSLMPSDISSAIEAQNTQVSAGKIGAQPVGGDQQLTATVTAQSR
LKTPAQFRDIIVKSESSGAMVRLGDVARVELGNEDYTTATRLNGHPAGGIAVMLSSGANA
LATAERVKAKVAEFTPQMPQGYEVSYPKDSTDFIKVSVEEVVYTLFEAIALVVLVMFLFL
QNLRATLIPAVAVPVVLLGTFGVLAVFGYSINTLTLFGMVLAIGLLVDDAIVVVENVERL
MRDQGLSPVAATEQSMREISSALVGVALVLSAVFLPMAFFGGSTGVIYRQFSITIVSSML
LSVVVALTLTPALCAALLKPVAHAPSNGNGFFARFNRGYENLQQRYTGRVAHVITGPWRY
LGLYGLLIVVMGVLFLRLPGGFLPNEDQGDVMVQFTLPAGATETRTSKVGERIEHYFLSE
EKDNVEAIFVISGFNFSGSGQNAGMAFVALKNWSLRPGSANSSTAIARRAMQALSAIRDA
QVFTMSPPAVQGLGQSDGFTFELQATGNTDRSRLLALRDQLLAEAAKNPRLAAVRPNDLV
QMPQLQVDLDYAKIRALGLSISDVTSTLSSAWGGTYVNDFIDRGRVKKVYIQGDAGSRGQ
PSDLDKWFVRGADSSGNSVMTPFSAFAATRWSYGPESLSRYNGLASYELQGTGAPGVSSG
KAMDEMEALARALPAGTTFAWSELSYQERLASGQAISLYAMSILVVFLCLAALYESWSIP
FSVMMVIPLGVVGAVLAATVRGLENDVYFQVALLTTIGLSAKNAILIVEFAEAAYRRGAT
LYEAALEGARTRLRPVLMTSMAFIAGVFPLAVATGAGANSRISIGSGIVGGTLTATVLAI
FLVPMFFVLVRRVFPGRHTHTLPSTSTPTQGEN