Protein Info for DZA65_RS14185 in Dickeya dianthicola ME23

Annotation: PfaD family polyunsaturated fatty acid/polyketide biosynthesis protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 transmembrane" amino acids 64 to 84 (21 residues), see Phobius details amino acids 130 to 139 (10 residues), see Phobius details TIGR02814: PfaD family protein" amino acids 30 to 468 (439 residues), 660.8 bits, see alignment E=4.6e-203 PF21607: FabD_helical_ins" amino acids 345 to 424 (80 residues), 107.6 bits, see alignment E=1.5e-35

Best Hits

KEGG orthology group: None (inferred from 91% identity to dda:Dd703_1432)

MetaCyc: 44% identical to icosapentaenoate synthase D subunit (Shewanella pneumatophori)
2.3.1.-

Predicted SEED Role

"Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39); Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9)" (EC 1.3.1.9, EC 2.3.1.39)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.9, 2.3.1.39

Use Curated BLAST to search for 1.3.1.9 or 2.3.1.39

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CDI2 at UniProt or InterPro

Protein Sequence (481 amino acids)

>DZA65_RS14185 PfaD family polyunsaturated fatty acid/polyketide biosynthesis protein (Dickeya dianthicola ME23)
MTHLGQTTRAERGVSLPLQGGGSRQGPLVQAEQLGSTAFRQDYGIKYAYVSGAMYQGIAS
RKLVVAMARAGMMSFFGSGGVSLGVLEDAILGIQSDLSGESFGMNLLADYDFPAKEMQRV
ELFLTHGVRFIEAAAYIAITPALVRYRLSGVHRGDDQRIIIPNTLMAKVSRPEVADVFAH
PAPPELVAGLCQQGLITEQEAELAPLVPMAQDICVESDSAGHTDQGNAYVLFPAISRLCR
DVSARQRYQKPIRVGAAGGIGTPEAVMAAFMLGADFIVTGSVNQCSVEAATSDEVKDLLQ
SINIQDTDYAPAGDMFEQGAKVQVLRRGVFFPSRANKLYQLYQQYASLEAIPQVVKKQLE
DKYFKRPLSDVWQDVRQYYQANRPQMLAKIEEHPKRKMAAVFRWYFAYSSRLALAGDREN
QVDYQVHCGPALGAFNQWVKGSAYESWRARHVDEIGEMLMQEAALMLSRQIGLITSHSLS
S