Protein Info for DZA65_RS13970 in Dickeya dianthicola ME23

Annotation: flagellar assembly peptidoglycan hydrolase FlgJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 316 TIGR02541: flagellar rod assembly protein/muramidase FlgJ" amino acids 12 to 298 (287 residues), 345.9 bits, see alignment E=1.4e-107 PF10135: Rod-binding" amino acids 51 to 98 (48 residues), 61 bits, see alignment 1.3e-20 PF01832: Glucosaminidase" amino acids 163 to 298 (136 residues), 119.2 bits, see alignment E=1.7e-38

Best Hits

KEGG orthology group: K02395, flagellar protein FlgJ (inferred from 96% identity to ddd:Dda3937_02211)

Predicted SEED Role

"Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-)" in subsystem Flagellum (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y143 at UniProt or InterPro

Protein Sequence (316 amino acids)

>DZA65_RS13970 flagellar assembly peptidoglycan hydrolase FlgJ (Dickeya dianthicola ME23)
MSDMKTLNNPAYETQSLNALKRDVSSHPQSREGIRAVAKQLEGVFVQMMMKSMRDALPKD
GIFSSDQTRMFTSMYDQQLTQQLSSGKGLGLASVIEKQMMGQGIAGNEPASAAPFKPDGQ
LARAMPSFVLEQMVRKAMPTLPEKSSSLPMSSTDFISRLSTPAMLVSQQSGIPHHLIMAQ
AALESGWGQREIPTADGRRSHNIFGIKAGSSWDGPITEVTTTEYEQGVAKKVKAAFRVYG
SYLEALNDYARLLTQNPRYAGVSAAATAEQAAVALQQAGYATDPAYAKKLVSMIQQMKNS
GEKAVQAYTRDLSGIF