Protein Info for DZA65_RS12465 in Dickeya dianthicola ME23

Annotation: nitrate reductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1257 transmembrane" amino acids 202 to 220 (19 residues), see Phobius details TIGR01580: nitrate reductase, alpha subunit" amino acids 2 to 1237 (1236 residues), 2409.5 bits, see alignment E=0 PF14710: Nitr_red_alph_N" amino acids 3 to 40 (38 residues), 62.5 bits, see alignment (E = 5.4e-21) PF00384: Molybdopterin" amino acids 108 to 834 (727 residues), 239.1 bits, see alignment E=1.2e-74 PF01568: Molydop_binding" amino acids 1089 to 1203 (115 residues), 69.8 bits, see alignment E=3.1e-23

Best Hits

Swiss-Prot: 82% identical to NARG_ECOLI: Respiratory nitrate reductase 1 alpha chain (narG) from Escherichia coli (strain K12)

KEGG orthology group: K00370, nitrate reductase 1, alpha subunit [EC: 1.7.99.4] (inferred from 72% identity to aeh:Mlg_1003)

MetaCyc: 72% identical to respiratory nitrate reductase alpha subunit (Stutzerimonas stutzeri)
RXN-11236 [EC: 1.7.5.1]

Predicted SEED Role

"Respiratory nitrate reductase alpha chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.5.1 or 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4DDL9 at UniProt or InterPro

Protein Sequence (1257 amino acids)

>DZA65_RS12465 nitrate reductase subunit alpha (Dickeya dianthicola ME23)
MSKFLDRLRYFKQVAEPFSGDHGQTLNTNRDWEDGYRSRWQHDKVVRSTHGVNCTGSCSW
KIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWYLYSANRLKYPMMRKRLMK
LWREARQHHADPVDAWASIVGDASKAQEYKKVRGRGGFVRADWQEVNELIAAANVYTAKT
FGPDRVIGFSPIPAMSMVSYAAGARYLSLLGGVCLSFYDWYCDLPPASPQTWGEQTDVPE
SADWYNSSYIIAWGSNVPQTRTPDAHFFAEVRYKGTKTVAITPDYAEIAKLCDQWLNPKQ
GTDSALALAMGHVILNEFHLKQPRQYFTDYVRRYTDMPMLVLLEARADGSYAAGRLLRAG
DLVGNLGQENNPEWKTVAIDEPTGELVAPQGSIGYRWGEQGKWNLEQREGGAQRDVNLQL
SLLGKHDEVVSVGFPYFGGASSENFAGVALDEVLLHQLPVKRLTLADGQQALVTSVYDLT
LANYGVDRGLDDAQCGTDYDQVKAYTPAWAEQITGVSRHNIIRIAREFADNADKTHGRSM
IIVGAGMNHWYHLDMNYRGIINMLIFCGCVGQSGGGWAHYVGQEKLRPQTGWLPLAFGLD
WQRPPRHMNSTSFFYNHSSQWRYETIAPQELLSPLADSSRFSGSLIDFNVRAERMGWLPS
APQLGANPLRIAEQAAAAGMSPQDYTVAELKAGRLGFAAEQPDNPQNFPRNLFIWRSNLL
GSSGKGHEYMLKYLLGTEHGIQGQDLGQQGGVTPEEVEWREQGGEGKLDLVVTLDFRMSS
TCLYSDIVLPTATWYEKDDMNTSDMHPFIHPLSAAVDPAWESKTDWEIYKGLAKTFSRVC
QGHLGVETDVVTLPIQHDSAAEMAQPLGVKDWKKGECDLIPGKTAPHIMTVERDYPNLYA
RFTSLGPLLDKLGNGGKGISWNTQNEVDFLKRLNRVQEDGPAAGRPRIDTAIDAAEVILS
LAPETNGQVAVKAWEALGKVTGRDHTHLAHPKEDEKIRFRDIQAQPRKIISSPTWSGLED
EHVSYNACYTNVHELIPWRTISGRQQLYQDHEWMRAFGESLLVYRPPVDTRAAQPLLNKK
PNGNPEKALNFLTPHQKWGIHSTYSENLLMLTLGRGGPIVWLSEDDARELTIADNDWVEV
FNANGALTARAVVSQRIPSGMTMMYHAQERLVNLPGSEVTGQRGGIHNSVTRVCPKPTHM
IGGYAQLAYGFNYYGTVGSNRDEFVVVRKMNRIDWLDEDSHDDLSSHVQPHLQEKAR