Protein Info for DZA65_RS12100 in Dickeya dianthicola ME23

Annotation: ShlB/FhaC/HecB family hemolysin secretion/activation protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 558 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF08479: POTRA_2" amino acids 76 to 151 (76 residues), 84.6 bits, see alignment E=5.1e-28 PF17287: POTRA_3" amino acids 152 to 204 (53 residues), 79.9 bits, see alignment 1.2e-26 PF03865: ShlB" amino acids 209 to 521 (313 residues), 331.8 bits, see alignment E=7.7e-103

Best Hits

Swiss-Prot: 96% identical to CDIB_DICD3: Outer membrane transporter CdiB (cdiB) from Dickeya dadantii (strain 3937)

KEGG orthology group: None (inferred from 96% identity to ddd:Dda3937_04154)

Predicted SEED Role

"Channel-forming transporter/cytolysins activator of TpsB family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XXZ9 at UniProt or InterPro

Protein Sequence (558 amino acids)

>DZA65_RS12100 ShlB/FhaC/HecB family hemolysin secretion/activation protein (Dickeya dianthicola ME23)
MPVSSRKTGLMVCFFLIGYTASAFSAPLNPAERNETQQRQSEVIEQSRQQREALQQLNNI
VQAPLKADNAPQGPCFTLREIRFNHSTLLRLRDQTALTAGYLNRCNNLEQINQLVHDVSN
WYIQRGYITSRAFLSEQDLSGGVLQLEILEGRLEKITINNQTERMTRTAFPAREGEILNL
RDIEQGMEQMNRMPSQQVTIEIQPGSQPGYSVVNLTREAHLPFTGGVTFDNSGQKSTGEQ
QLNGSLALDNLFGVADQWFISAGHSSRFATSHDAESLQAGVSMPYGYWSLGYSYSQSRYR
NTFISRDFPWHSTGDSDTHRLSLSRVVFRNGTMKTAIAGTFSQRTGNNYLNGTLLPSSSR
KLNSVALGVNHSQKLWGGLATFNPTYNRGVRWLGAETDTDKSADEPRAEFNKWTLSASYY
YPVTDNITYLGSLYGQYSARALYGSEQLTLGGESSVRGFREQYTSGNRGAYWRNELNWQA
WQLPVLGRVTFMAAVDGGHLYNHKQDDATAASLWGGAVGVTVASRWLSQQVTVGWPISYP
AWLQPDTMVVGYRVGLSF