Protein Info for DZA65_RS11775 in Dickeya dianthicola ME23

Annotation: ammonia-dependent NAD(+) synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 88% identical to NADE_PECCP: NH(3)-dependent NAD(+) synthetase (nadE) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K01916, NAD+ synthase [EC: 6.3.1.5] (inferred from 96% identity to ddd:Dda3937_03367)

MetaCyc: 78% identical to NH3-dependent NAD+ synthetase (Escherichia coli K-12 substr. MG1655)
NAD(+) synthase. [EC: 6.3.1.5]

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

aspartate superpathway (24/25 steps found)
NAD de novo biosynthesis I (5/6 steps found)
NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
NAD salvage pathway I (PNC VI cycle) (5/7 steps found)
NAD de novo biosynthesis III (4/6 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4DBU0 at UniProt or InterPro

Protein Sequence (274 amino acids)

>DZA65_RS11775 ammonia-dependent NAD(+) synthetase (Dickeya dianthicola ME23)
MSLQQEIIQALGAKSTIDPAQEVRVSVDFLKNYLKAHPFVKSLVLGLSGGQDSTLTGKLC
QTAIAELRAETGKTDYQFIAVRLPYGVQADEQDCQDAIQFIQPDRVLTVNIKPAVDASEA
TLRAIGIELSDFVKGNEKARERMKAQYSIAGMNAGLVVGTDHAAEAVTGFFTKYGDGGTD
INPIFRLNKRQGKALLALLGCPAHLYTKAPTADLEDDRPSLPDEAALGVTYEKIDDYLEG
KQLAPVDAAIIESWYRRTEHKRRSPVTVFDDFWH