Protein Info for DZA65_RS11725 in Dickeya dianthicola ME23

Annotation: pectate disaccharide-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 543 PF06917: Pectate_lyase_2" amino acids 40 to 520 (481 residues), 679.4 bits, see alignment E=1.5e-208

Best Hits

Swiss-Prot: 96% identical to PLYW_DICD3: Pectate disaccharide-lyase (pelW) from Dickeya dadantii (strain 3937)

KEGG orthology group: K01731, pectate disaccharide-lyase [EC: 4.2.2.9] (inferred from 96% identity to ddd:Dda3937_03361)

MetaCyc: 96% identical to exopolygalacturonate lyase W (Dickeya dadantii 3937)
Pectate disaccharide-lyase. [EC: 4.2.2.9]; 4.2.2.9 [EC: 4.2.2.9]

Predicted SEED Role

"Exopolygalacturonate lyase (EC 4.2.2.9)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 4.2.2.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.2.9

Use Curated BLAST to search for 4.2.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y176 at UniProt or InterPro

Protein Sequence (543 amino acids)

>DZA65_RS11725 pectate disaccharide-lyase (Dickeya dianthicola ME23)
MSIFTDLNTSRKWQIEQWLSAINNHIEKIQQYGHSVVNPTPLLADGFEIKTQSPVVWQFP
DGHDAPISNFASQQNWLRLLISMSAVTETEKYRQLAFSQSEYFLNRFVDENSGLFYWGGH
RFINLDTLVSEGPESKSMVHELKHHLPYYEFLHQVNSEKTRHFIQGFWNAHVEDWNSLDL
GRHGDYARQRDPDVFLHSRRDVVTPAKWPELPLTKGLTFVNAGTDLIYAAFVYARHTGDV
HAAAWGKHLYRQYVLARNPETGMPVYQFSSPLPRQPVPVDDNQTQSWFGDRAQRQFGTEF
GAIAREANVLFRDMRPLLIDNPLAMLDILRHQPDAEILTWVIAGLKNYYQYAYDVDSNSL
RPMWNNGQDMTGYCFKRDGYYGKAGTVLKPFPLEGDYLLPLVRAWRLSDDDDLYTLIVTM
LSRLEKQGIHQSASPFLLLAITELAHAKQSAQWAEYAWQMAEILFKRYFHHGLFVRSEHH
RYVRLDDPFPAILLTLIAACRNKWPEIPAILTQGGYIHGDYRINGESRVIYDTEFIYPEK
LIH