Protein Info for DZA65_RS11160 in Dickeya dianthicola ME23

Annotation: bifunctional NADP-dependent 3-hydroxy acid dehydrogenase/3-hydroxypropionate dehydrogenase YdfG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 249 PF00106: adh_short" amino acids 2 to 187 (186 residues), 181.4 bits, see alignment E=3.5e-57 PF01370: Epimerase" amino acids 3 to 135 (133 residues), 26.4 bits, see alignment E=1.1e-09 PF08659: KR" amino acids 4 to 159 (156 residues), 47.1 bits, see alignment E=6.6e-16 PF13561: adh_short_C2" amino acids 7 to 220 (214 residues), 122 bits, see alignment E=7.6e-39 PF03807: F420_oxidored" amino acids 12 to 48 (37 residues), 21.7 bits, see alignment 6.6e-08

Best Hits

Swiss-Prot: 74% identical to YDFG_SALTY: NADP-dependent 3-hydroxy acid dehydrogenase YdfG (ydfG) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 93% identity to dze:Dd1591_2130)

MetaCyc: 74% identical to 3-hydroxy acid dehydrogenase YdfG (Escherichia coli K-12 substr. MG1655)
RXN-16000 [EC: 1.1.1.381]; RXN-8974 [EC: 1.1.1.381, 1.1.1.298]; Serine 3-dehydrogenase. [EC: 1.1.1.381, 1.1.1.298, 1.1.1.276]

Predicted SEED Role

"3-hydroxypropionate dehydrogenase (EC 1.1.1.298)" (EC 1.1.1.298)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.276 or 1.1.1.298 or 1.1.1.381

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4C4X3 at UniProt or InterPro

Protein Sequence (249 amino acids)

>DZA65_RS11160 bifunctional NADP-dependent 3-hydroxy acid dehydrogenase/3-hydroxypropionate dehydrogenase YdfG (Dickeya dianthicola ME23)
MIIFVTGATAGFGEAITRRFIKEGHQVIATGRRQDRLDALKDEFGAALLTLRLDVRDRQA
IEQAIASLPAEWRNIDVLVNNAGLALGLEPAHRASADDWETMIDTNNKGLVFMTHALLPE
MVKRNAGHVINIGSTAGNWPYLGGNVYGASKAFVRQFSLGLRADLSGTRVRVTNIEPGLV
GGTEFSAVRFKGDEAKVSQTYDKSNPLTAEDVTEAVFWVATLPSHVNINTLEMMPVSQTY
GGLKVNRDE