Protein Info for DZA65_RS10830 in Dickeya dianthicola ME23

Annotation: UTP--glucose-1-phosphate uridylyltransferase GalU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01099: UTP--glucose-1-phosphate uridylyltransferase" amino acids 8 to 275 (268 residues), 377.2 bits, see alignment E=2.2e-117 PF00483: NTP_transferase" amino acids 10 to 277 (268 residues), 102.3 bits, see alignment E=1.6e-33

Best Hits

Swiss-Prot: 84% identical to GALU_ECO57: UTP--glucose-1-phosphate uridylyltransferase (galU) from Escherichia coli O157:H7

KEGG orthology group: K00963, UTP--glucose-1-phosphate uridylyltransferase [EC: 2.7.7.9] (inferred from 99% identity to ddc:Dd586_2147)

MetaCyc: 84% identical to UTP--glucose-1-phosphate uridylyltransferase (Escherichia coli K-12 substr. MG1655)
UTP-monosaccharide-1-phosphate uridylyltransferase. [EC: 2.7.7.64, 2.7.7.9]

Predicted SEED Role

"UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9)" (EC 2.7.7.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.9

Use Curated BLAST to search for 2.7.7.64 or 2.7.7.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XY57 at UniProt or InterPro

Protein Sequence (303 amino acids)

>DZA65_RS10830 UTP--glucose-1-phosphate uridylyltransferase GalU (Dickeya dianthicola ME23)
MSTVNKKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQYVVNECIAAGINEIILV
THSSKNSIENHFDTSFELEAMLEKRVQRQLLKEVQSICPEHVTIMQVRQGLAKGLGHAVL
CAHPLVGDEPVAVILPDVIIDEYESDLKKDNLSEMLQRFYSTGHSQIMVEPVENVSSYGV
VDCKGVELKPGDSAPMVGVVEKPKASEAPSNLAVVGRYVLSEQIWDLLKKTEPGAGNEIQ
LTDAIAMLMEKETVEAYHLKGVSHDCGNKLGYMTAFVEYGIRHDALGEDFTQWLKEAIEE
DAN