Protein Info for DZA65_RS10220 in Dickeya dianthicola ME23

Annotation: tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 273 PF01209: Ubie_methyltran" amino acids 26 to 154 (129 residues), 32 bits, see alignment E=2.5e-11 PF13489: Methyltransf_23" amino acids 38 to 152 (115 residues), 49.8 bits, see alignment E=1e-16 PF13847: Methyltransf_31" amino acids 46 to 151 (106 residues), 54.1 bits, see alignment E=4.6e-18 PF13649: Methyltransf_25" amino acids 48 to 143 (96 residues), 63.3 bits, see alignment E=8.4e-21 PF08242: Methyltransf_12" amino acids 49 to 145 (97 residues), 58.6 bits, see alignment E=2.6e-19 PF08241: Methyltransf_11" amino acids 49 to 146 (98 residues), 67.5 bits, see alignment E=4e-22

Best Hits

Swiss-Prot: 64% identical to CMOM_ECOLI: tRNA 5-carboxymethoxyuridine methyltransferase (cmoM) from Escherichia coli (strain K12)

KEGG orthology group: K06219, S-adenosylmethionine-dependent methyltransferase (inferred from 95% identity to dze:Dd1591_2302)

MetaCyc: 64% identical to tRNA cmo5U34 methyltransferase (Escherichia coli K-12 substr. MG1655)
2.1.1.M28 [EC: 2.1.1.M28]

Predicted SEED Role

"S-adenosylmethionine-dependent methyltransferase Functionally Coupled to the MukBEF Chromosome Partitioning Mechanism" in subsystem MukBEF Chromosome Condensation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.M28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y1U6 at UniProt or InterPro

Protein Sequence (273 amino acids)

>DZA65_RS10220 tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM (Dickeya dianthicola ME23)
MQDRNFNDIADKFARNIYGTTKGRLRQAVLWQDLEILLTRLPSQPLRILDAGGGEGPMSR
RLAALGHQVLLCDLSDEMIARAREAAQEQGVADNMRFVCCAAQDIGAHLSQPVDLVLFHA
VLEWVAQPQQALRALTDCLAPGGALSLMFYNHQALVMRNMVLGNFGYVDAGMPKRKRRSL
SPDHPLDPPQVYQWLDDLGLALSGKTGIRVFHDYLQNKQQQIDDFDLLLALEQRYCRQEP
FVSLGRYIHVMAHKHVMAHKHVMAHKPPMKDAL