Protein Info for DZA65_RS10180 in Dickeya dianthicola ME23

Annotation: lipid A ABC transporter ATP-binding protein/permease MsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 582 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details amino acids 41 to 41 (1 residues), see Phobius details transmembrane" amino acids 40 to 40 (1 residues), see Phobius details amino acids 64 to 93 (30 residues), see Phobius details amino acids 142 to 163 (22 residues), see Phobius details amino acids 168 to 186 (19 residues), see Phobius details amino acids 245 to 269 (25 residues), see Phobius details amino acids 276 to 295 (20 residues), see Phobius details TIGR02203: lipid A export permease/ATP-binding protein MsbA" amino acids 12 to 581 (570 residues), 865.1 bits, see alignment E=1.2e-264 PF00664: ABC_membrane" amino acids 29 to 298 (270 residues), 207.3 bits, see alignment E=5.6e-65 PF00005: ABC_tran" amino acids 359 to 509 (151 residues), 122.3 bits, see alignment E=3.4e-39

Best Hits

Swiss-Prot: 86% identical to MSBA_PECAS: Lipid A export ATP-binding/permease protein MsbA (msbA) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K11085, ATP-binding cassette, subfamily B, bacterial MsbA [EC: 3.6.3.-] (inferred from 97% identity to dze:Dd1591_2309)

MetaCyc: 82% identical to ATP-binding lipopolysaccharide transport protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-236 [EC: 7.5.2.6]; 7.5.2.6 [EC: 7.5.2.6]

Predicted SEED Role

"Lipid A export ATP-binding/permease protein MsbA (EC 3.6.3.25)" in subsystem KDO2-Lipid A biosynthesis or Lipopolysaccharide-related cluster in Alphaproteobacteria (EC 3.6.3.25)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-, 3.6.3.25

Use Curated BLAST to search for 3.6.3.- or 3.6.3.25 or 7.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XXK8 at UniProt or InterPro

Protein Sequence (582 amino acids)

>DZA65_RS10180 lipid A ABC transporter ATP-binding protein/permease MsbA (Dickeya dianthicola ME23)
MINDKDLSTWQTFRRLWPMISPYKAGLTVAAVALVVNAAGDTLMLSLLKPLLDDGFGKAD
RSVLVWMPLAIIGLMIMRGISSYISSYCVAWVSGKVVMNMRRRLFSHIMGMPVSFFDQQS
TGTLLSRITYDSEQVAASSSNALVTVVREGASIIGLFVLMFWYSWQLSLILVLIAPIVSY
SMRVVSKRFRSISKSMQNTMGQVTTSAEQMLKGHKEVLIFGGQTVEVERFDAVSNRMRQQ
GMKMVSASSISDPVVQLIASLALAFVLYAASFPSVMETLTAGTITVVFSSMFTLMRPLKS
LTNVNAQFQRGMAACQTLFTILDMEQEKDTGTRVLAHAKGNIEFRQVNFTYPGKETLALK
NISLHIPPGKTVALVGRSGSGKSTIANLITRFYDIQAGEILLDGHDLRDYTLSSLRSQVA
LVSQNVHLFNDTIANNIAYACGDRYSREDIERAAKMAHAMDFIGRLEQGLDTVIGENGVL
LSGGQRQRIAIARALLRDCPILVLDEATSALDTESERAIQAALDELQKNRTALVIAHRLS
TIEKADEILVVEDGQIIERGQHSELLARNGAYAQLHKLQFGA