Protein Info for DZA65_RS09745 in Dickeya dianthicola ME23

Annotation: D-alanyl-D-alanine carboxypeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF00768: Peptidase_S11" amino acids 34 to 267 (234 residues), 322.2 bits, see alignment E=3.1e-100 PF13354: Beta-lactamase2" amino acids 46 to 199 (154 residues), 50.3 bits, see alignment E=3.2e-17 PF07943: PBP5_C" amino acids 287 to 378 (92 residues), 92.1 bits, see alignment E=2.9e-30

Best Hits

Swiss-Prot: 70% identical to DACC_ECOLI: D-alanyl-D-alanine carboxypeptidase DacC (dacC) from Escherichia coli (strain K12)

KEGG orthology group: K07258, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5/6) [EC: 3.4.16.4] (inferred from 96% identity to ddd:Dda3937_03486)

MetaCyc: 70% identical to D-alanyl-D-alanine carboxypeptidase DacC (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.4.16.4, 3.4.17.8]

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4 or 3.4.17.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4D1M1 at UniProt or InterPro

Protein Sequence (402 amino acids)

>DZA65_RS09745 D-alanyl-D-alanine carboxypeptidase (Dickeya dianthicola ME23)
MKIVTPRISWGRVVVVAATALMTVSPVLRAEQMPPPPQIEAKAFVLIDSASGRVLAESNA
DARLDPASLTKIMSSYVIGQAIKVGKITPEDKVTVGKDAWATGNPALRGSSLMFLKPGDQ
IPVSDLNKGMVIQSGNDASIALADYVAGSQDAFVSLMNQYAATLGLANTHFLTVHGLDTL
GQYSTARDMARLTQALIRDVPGEYALHSEKEFTFNNIRQPNRNRLLWSGNLRVDGVKTGY
TGGAGHSLVASAVDSDMRLISVVLGAPSDAVRFRESEKLLTWGFRFFETVTPVRSDTPFT
TPRVWFGTEKQARLSVAQDAALTVPKGQMKNLKASFTLAQPQLTAPLKKGQVVGTIDFQL
NGNSIGQRPLVVMEDIPEAGFFSRLWDRVLMTLSGWFGGVFG