Protein Info for DZA65_RS09565 in Dickeya dianthicola ME23

Annotation: fused PTS fructose transporter subunit IIA/HPr protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 PF00359: PTS_EIIA_2" amino acids 4 to 139 (136 residues), 102.6 bits, see alignment E=1.8e-33 TIGR01003: phosphocarrier, HPr family" amino acids 290 to 370 (81 residues), 74.8 bits, see alignment E=2e-25 PF00381: PTS-HPr" amino acids 291 to 371 (81 residues), 89.3 bits, see alignment E=1.4e-29

Best Hits

Swiss-Prot: 68% identical to PTFAH_SALTY: Multiphosphoryl transfer protein (fruB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K11183, phosphocarrier protein FPr (inferred from 95% identity to dze:Dd1591_2426)

MetaCyc: 67% identical to fructose-specific PTS multiphosphoryl transfer protein FruB (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Fructose-specific phosphocarrier protein HPr (EC 2.7.1.69) / PTS system, fructose-specific IIA component (EC 2.7.1.69)" in subsystem Fructose utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CN11 at UniProt or InterPro

Protein Sequence (379 amino acids)

>DZA65_RS09565 fused PTS fructose transporter subunit IIA/HPr protein (Dickeya dianthicola ME23)
MFQLSQHDIHLGASAGSKEEAIRQVAAALTEAGCVSEGYVEGMLQRELQTSTYLGSGIAI
PHGTTDTRDLVRKTGVQVFQFPQGIAWGEDQTAYVVLGIAARSDEHLALLRQLTHVLSDD
RVAARLASTTSADELRGLLMGEQLAGSFRFDTSLISLNVATDNLMTLQALNAGRLQQIGA
VDAGFVSHVVGNKPLHLGQGVWLSDSLNGNRVSALAVSRPAEPFAVDGESVALLLTVSAA
DEQVFASLDYLSKLLIAGKADSLLSADEATLLALLTSEEEEEDESDALVAEFTIRNEHGL
HARPGAMLVNVIKQFSSEITVTNLDGTGKPANGRSLMKVVALGVKSGHRLRFTAKGSDAQ
AALKAIGDVIQSGLGEGAA