Protein Info for DZA65_RS09150 in Dickeya dianthicola ME23

Annotation: beta-glucosidase BglX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 777 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 58 to 363 (306 residues), 291 bits, see alignment E=2e-90 PF01915: Glyco_hydro_3_C" amino acids 407 to 660 (254 residues), 204.3 bits, see alignment E=3.2e-64 PF14310: Fn3-like" amino acids 698 to 766 (69 residues), 86.2 bits, see alignment E=2e-28

Best Hits

Swiss-Prot: 74% identical to BGLX_ECOLI: Periplasmic beta-glucosidase (bglX) from Escherichia coli (strain K12)

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 95% identity to dze:Dd1591_2497)

MetaCyc: 74% identical to beta-D-glucoside glucohydrolase, periplasmic (Escherichia coli K-12 substr. MG1655)
Beta-glucosidase. [EC: 3.2.1.21]

Predicted SEED Role

"Periplasmic beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XWJ7 at UniProt or InterPro

Protein Sequence (777 amino acids)

>DZA65_RS09150 beta-glucosidase BglX (Dickeya dianthicola ME23)
MKWFTSLALAVGLACSPLWAQTPGSMLQQTALQQTSPQAASARQQRDAFVNDLMKKMTLD
EKIGQLRLISVGPDNPKHAIREMIRNGQVGGIFNTVTRQDIRAMQDQVMQLSRLKIPLFF
AYDVVHGQRTVFPIGLGLASSWDMSTVELSAKIAAYEATEDGLNMTWAPMVDITRDPRWG
RVSEGFGEDTYLTSEIARVMVKGFQGDDLTGRHSLMTSVKHYALYGAAEGGRDYNTVDMS
PQRMFQDYMPPYKAAIDAGSYGVMASLNSINGVPATANRWLLKDVLRDQWHFKGITISDH
GAIKELIKHGVAADPSDASRIAVQSGIGMSMSDEYFVRYLPDLVKRGLVSMKDIDDACRQ
VLNMKYDMGLFEDPYNHLGPVGSDPVDTNAESRLHREEARDVARRSLVLLKNRLDILPLK
KSGAIAVVGPLADSKRDIIGSWSAAGRKAQAVTVYEGIRKAVGYNARVYYAKGSNVTNHP
GLLKFLNEYDESVVVDPRSPQAMIDEAVDVAKKSDVVIAVVGESQGMAHEASSRAKITIP
PEQKALISALKATGKPLVLVLMNGRPLDLSREDQQANALLETWFSGTEGGNAIADVLFGD
YNPSGKLPMTFPRSVGQIPMYYSHLPTGRPYSAEAPNKYTSHYFDEANGPLYPFGYGLSY
TTFEVSDVKLSSPTMKRNGSVKASVTVTNTGKRAGETVAQLYLHDVVASVSRPVKELRGF
QKVMLLPGESRTLTFTLSPQDLLFYNAQMQQVAEPGKFEVMIGLDSQRVKTGSFTLL