Protein Info for DZA65_RS08700 in Dickeya dianthicola ME23

Annotation: ABC transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 signal peptide" amino acids 9 to 9 (1 residues), see Phobius details transmembrane" amino acids 10 to 30 (21 residues), see Phobius details amino acids 101 to 125 (25 residues), see Phobius details amino acids 137 to 156 (20 residues), see Phobius details amino acids 176 to 195 (20 residues), see Phobius details amino acids 232 to 257 (26 residues), see Phobius details amino acids 277 to 298 (22 residues), see Phobius details PF19300: BPD_transp_1_N" amino acids 2 to 105 (104 residues), 44.3 bits, see alignment E=1.8e-15 PF00528: BPD_transp_1" amino acids 117 to 307 (191 residues), 129.2 bits, see alignment E=1.5e-41

Best Hits

Swiss-Prot: 41% identical to NIKB_ECOLI: Nickel transport system permease protein NikB (nikB) from Escherichia coli (strain K12)

KEGG orthology group: K02033, peptide/nickel transport system permease protein (inferred from 91% identity to ddd:Dda3937_00761)

MetaCyc: 41% identical to nickel ABC transporter membrane subunit NikB (Escherichia coli K-12 substr. MG1655)
7.2.2.i [EC: 7.2.2.i]; 7.2.2.- [EC: 7.2.2.i]

Predicted SEED Role

"Nickel transport system permease protein NikB (TC 3.A.1.5.3)" in subsystem Transport of Nickel and Cobalt (TC 3.A.1.5.3)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.2.2.i

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CM08 at UniProt or InterPro

Protein Sequence (310 amino acids)

>DZA65_RS08700 ABC transporter permease subunit (Dickeya dianthicola ME23)
MRAYFIKRLFMTLPLAVVMSFVAFSLLNLVPSDPAEVALRVNDIVPTDEAIQLMHHELGL
DRPFFPRYVLWLWKVLHGDFGTSFITRQPVWHVFMQALPATLYLAAVSLLMIVTLSLTLG
IACALTENTPFDKAVRSVVFLTVAIPNYWIGLLLMWGLAVNMDLFPVGGMQEERSVILPS
CTLMLGYIGTYIRLIRGSMIGNLQQNYVLYARARGLPAPLIIGKHVLANSLHAALVAIGM
SIPKLIAGTVVIENIFAWPGVGRLCLSAIFNRDYPMIQAYMLLMALLFLGFNFLTDVLQA
KLDPRIRGER