Protein Info for DZA65_RS08195 in Dickeya dianthicola ME23

Annotation: alpha-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 709 PF16875: Glyco_hydro_36N" amino acids 24 to 245 (222 residues), 157.2 bits, see alignment E=8.6e-50 PF02065: Melibiase" amino acids 255 to 593 (339 residues), 382.5 bits, see alignment E=2.7e-118 PF16874: Glyco_hydro_36C" amino acids 611 to 695 (85 residues), 34.7 bits, see alignment E=2.9e-12

Best Hits

Swiss-Prot: 70% identical to RAFA_ECOLX: Alpha-galactosidase (rafA) from Escherichia coli

KEGG orthology group: K07407, alpha-galactosidase [EC: 3.2.1.22] (inferred from 95% identity to dze:Dd1591_2658)

Predicted SEED Role

"Alpha-galactosidase (EC 3.2.1.22)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Melibiose Utilization (EC 3.2.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XVM5 at UniProt or InterPro

Protein Sequence (709 amino acids)

>DZA65_RS08195 alpha-galactosidase (Dickeya dianthicola ME23)
METTLIRLASPATDVIVRCWPAAEILYWGPHLAAFSPQDAQSLSRPVANGRLDVDVPVTL
AAETGRGLFGSPGIEGHRNGLDAFAVLTTVAVRQPDPQQLVIEAEDSQAGLRLTSELRLH
ADSGVLQLRHCLTNLHPGDWQVQRLALTLPLPESAAEVMAFHGRWVREFQPHRTLLAHGS
LVQENRRGRTSHEYFPAMVSGEPGFAEQHGRVWGVHLGWSGNHRLRAEVKTDGRRLVQAE
ALYLPGEIALAQGESLTTPWLYAACSEQGLTGMSQQFHRFLRQEIIRFPHAKARPVHLNT
WEGIYFKHDPAYIMQMASQAAALGVERFIIDDGWFRGRDHDRAGLGDWYVDPRKYPQGLM
PVINHVRELGMEFGIWVEPEMINPDSDLCRAHPDWLLALPGYHQPTGRYQYVIDLSNPAA
FDYLLARLSWLLGEHPIDYVKWDMNRELVQPAHQGRAAADRQTQAFYRLLDVLGERFPQV
EFESCASGGGRIDYEVLRRCHRFWVSDNNDALERQTIQRGMSYFFPPEVMGAHIGHHRCH
ATGRQHSIAFRGLTALFGHMGIELDPVRADAQEQAGFRHYVALHRRYRALLHTGSLWRAD
MADAATQVQGVISADRRQALFLVAQLAMPDYALAGVLRFPGLDAQARYRLTVVDHPDLKP
VIDGGSTMRQLPAWMAALAEYSGEWLMKAGLRLPILNPETALLLELERL