Protein Info for DZA65_RS08170 in Dickeya dianthicola ME23

Annotation: formate dehydrogenase-N subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01553: formate dehydrogenase-N alpha subunit
  • amino acids 2 to 1014 (1013 residues), 1527.7 bits, E=0
• PF04879: Molybdop_Fe4S4
  • amino acids 44 to 103 (60 residues), 55.1 bits, 9.2e-19
• PF00384: Molybdopterin
  • amino acids 107 to 658 (552 residues), 107.5 bits, E=1.1e-34
• PF01568: Molydop_binding
  • amino acids 903 to 1008 (106 residues), 45.4 bits, E=1.1e-15

Best Hits

Swiss-Prot: 70% identical to FDNG_ECOLI: Formate dehydrogenase, nitrate-inducible, major subunit (fdnG) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 97% identity to ddd:Dda3937_02963)

MetaCyc: 70% identical to formate dehydrogenase N subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase N alpha subunit (EC 1.2.1.2)" in subsystem Formate dehydrogenase or Formate hydrogenase (EC 1.2.1.2)

MetaCyc Pathways

• formate to dimethyl sulfoxide electron transfer (2/2 steps found)
• nitrate reduction III (dissimilatory) (2/2 steps found)
• formate oxidation to CO₂ (1/1 steps found)
• formate to nitrite electron transfer (2/3 steps found)
• formate to trimethylamine N-oxide electron transfer (1/2 steps found)
• oxalate degradation VI (2/4 steps found)
• oxalate degradation III (2/5 steps found)
• purine nucleobases degradation II (anaerobic) (13/24 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

See A0A385XVT8 at UniProt or InterPro

Protein Sequence (1015 amino acids)

>DZA65_RS08170 formate dehydrogenase-N subunit alpha (Dickeya dianthicola ME23)
MQVNRRGFFKVCAGGMAGTTLAVLGFTPTEAMASVRQYKLLRAKETRNNCTYCSVGCGLL
MYSLGDGAKNAKPSIFHIEGDPDHPVSRGSLCPKGAGLVDYIHSEGRLKYPEYRAPGSDK
WQRISWDDAIERIARLMKKDRDANFERLNAKGALVNRWLTTGMLCSSAASNETGVLDQKF
ARSLGMVAIDCQARLCHGPTVAALAPTFGRGAMTNNWVDIKNANVIIVMGGNAAEAHPVG
FKWAVEAKTHNDAKLIVVDPRFNRSAAVADLYAPIRAGSDAAFLLGVVNYLITHDKIHHE
YVKSYTSASLIVREDFSFDEGLFSGYNSQTHQYDKSSWQYELGADGFAKRDMTLSHPRCV
WNLLKKHVARYTPEMVTSLCGTPAKAYEEICQSLASTCVPNKTATFMYALGWTHHTNGAQ
IIRAAAMIQLLLGNIGMAGGGINALRGHSNIQGYTDLGLLSLNLPGYMPLPSEKQGDLKT
YLGQITPDALLADQVNYWKNTPKFFISMMKSFWGDHAQAANNWGYDWLPKWDRSYDVMAQ
TELMLDGKMNGYIVQGFNPLAAFSNKNKATAALSKLKYMVVIDPLATETSTFWQNHGEFN
DVNSAEIQTEVFRLPSSCFAEENGSIANSGRWLQWHWAAAEPPAEALHDAKILGRLMTRL
RELYREEGGVCPEPVLNINWNYQDPEDPTQEEIAREANGMALSDVFDDKGALLLKKGQQL
ADFSQLRDDGSTASFCWIYAGSWTEAGNQMANRDNTDVGLGCTPGWAWCWPQNRRILYNR
ASADLQGKPWDSKRKLLEWTGQKWKGIDVPDFAVTVPPGKDTMPFIMLPEGVARLFSLDK
LTDGPFPEHYEPIETPIGTNPLHPAVVSNPAARLFARDAKTMGKASDFPYVATTYSITEL
FRHWTKHARLNAIVQPEQFVEIGENLAKSKGIQAGDMVKVSCQRGYIKAKAVVTKRIKTL
TVAGKAIETVGIPCHWGFEGATRKGFLANTLTPSVGDANSQTPEYKAFLVNVEKV