Protein Info for DZA65_RS06280 in Dickeya dianthicola ME23

Annotation: phosphotransferase RcsD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 889 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 295 to 315 (21 residues), see Phobius details amino acids 336 to 353 (18 residues), see Phobius details PF00512: HisKA" amino acids 442 to 504 (63 residues), 27.8 bits, see alignment 4.4e-10 PF02518: HATPase_c" amino acids 553 to 665 (113 residues), 48.8 bits, see alignment E=1.7e-16 PF16359: RcsD_ABL" amino acids 675 to 776 (102 residues), 129.9 bits, see alignment E=8.1e-42 PF01627: Hpt" amino acids 801 to 881 (81 residues), 51.3 bits, see alignment E=2.2e-17

Best Hits

KEGG orthology group: K07676, two-component system, NarL family, sensor histidine kinase RcsD [EC: 2.7.13.3] (inferred from 96% identity to ddd:Dda3937_01777)

Predicted SEED Role

"Two-component sensor protein RcsD (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XUU4 at UniProt or InterPro

Protein Sequence (889 amino acids)

>DZA65_RS06280 phosphotransferase RcsD (Dickeya dianthicola ME23)
MPAAIVRFFLLFILLLLLMTGAYSYNYVNSWMTEKKSALTDIAEGMQKRIDAYRFSTDQI
YKTLISDPAPPDTPNINLITLMPNVFYAEKSGHKTDALIFGPHDKTTLGTMHRISQYLDV
LWGAKTDIYSMYYLNGQDNSLTVVSTQPLKDISSQFRGSYLSSMVESRKTEMLQQANTLD
ERESFSPLRKLRFYNDYYFTLRTTFNQPGHLATVIAFDLSINDLIPRNMPRDSFILRQQT
PPVNADANADNDIPTDIRREGALLEISAQLMNSPIKLVYAIPLGRLATDMLRNNIWAIAF
NLALLAISLVGFYTLRRYYARPKDDLSHHLKEQQRMYGEIVSRIPIGVLVYDFSNNKVMV
ANTLAERLYPHLSLKKISTLAEEHQGVIQATVDNEMYEVRVFHSQHTPSLCLFLLREQDQ
EILITKKLQLAQREFDKNVSVRKRLFRNLSQEFKQPLSAVHQLALTLRKTDNPPEQQKTV
QALIAETSSAIRLMENIALQARLETVEWHLVHDSFSPLTLIDDLMLELLPRIQQKGLKLF
NHYKLDPRQTYLGDSELLKKTLSLLLDYSITNTDYGKITLSCEPSAHSPEQLLIRISDTG
TDISGMERDNLVHPFATTPLSDRFRHNSGLTLFLCNQLCNKLGGQLQINSRPGLGTQYVL
TLKMQPDPLPAEEDEKLLDDITLLLNITSDEVRTIINRMVSNWGADIVVYDERLVSQDAE
ITITDDPSRMADDTLLVTSDDTLLVPLGHRRLRTNYNISQLLLEGLLKLIEQQLEASPAN
TLPEERDDIGFYVRQLRNSDYYSLFVDTVPEDLKRLYTETQDGDFLSLAQTAHRLKGVFA
MLNLHPGKQLCEALEKLITTQDRAQIETNLRQIEGFVTALLQPGGQQYE