Protein Info for DZA65_RS05410 in Dickeya dianthicola ME23

Annotation: LOG family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 PF14793: DUF4478" amino acids 4 to 110 (107 residues), 160.7 bits, see alignment E=1.6e-51 PF03641: Lysine_decarbox" amino acids 190 to 329 (140 residues), 97 bits, see alignment E=1.6e-31 PF11892: PpnN_C" amino acids 333 to 452 (120 residues), 194.9 bits, see alignment E=6.1e-62

Best Hits

Swiss-Prot: 84% identical to PPNN_ECO57: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase (ppnN) from Escherichia coli O157:H7

KEGG orthology group: K06966, (no description) (inferred from 98% identity to ddd:Dda3937_00870)

MetaCyc: 84% identical to nucleotide 5'-monophosphate nucleosidase (Escherichia coli K-12 substr. MG1655)
AMP nucleosidase. [EC: 3.2.2.4]; Inosinate nucleosidase. [EC: 3.2.2.4, 3.2.2.12]; Pyrimidine-5'-nucleotide nucleosidase. [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]; 3.2.2.- [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]

Predicted SEED Role

"Decarboxylase family protein"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.4

Use Curated BLAST to search for 3.2.2.10 or 3.2.2.12 or 3.2.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CC13 at UniProt or InterPro

Protein Sequence (454 amino acids)

>DZA65_RS05410 LOG family protein (Dickeya dianthicola ME23)
MITHISPLGSMDLLSQLEVDMLKSTASSDLYRLFRNCSLAVLNSGSQTDNSKELLSRYED
FDINVLRRERGVKLELVNPPEDAFVDGQIIRSLQANLFAVLRDILFVSGQIASAGRYQNL
DLEDSTHITNLVFSILRNARALHVGEEPNLVVCWGGHSINETEYLYARKVGSQLGLRELN
ICTGCGPGAMEAPMKGAAVGHAQQRYRDGRFIGMTEPSIIAAEPPNPLVNELIIMPDIEK
RLEAFVRIGHGIVIFPGGVGTAEEFLYLLGIMMDPDNSEQVLPIILTGPQESADYFQVLD
EFIVSTLGRQARRYYSIIINDAAEVARQMKKTMPQVKDHRRHTGDAYSFNWSLRIAPDLQ
LPFEPTHENMANLNLHPDQPAEQLAATLRRAFSGIVAGNVKEAGIRAIEEYGPYKLHGNA
DIMKSMDQLLRDFVSQQRMKLPGSAYVPCYEIRS