Protein Info for DZA65_RS05335 in Dickeya dianthicola ME23

Annotation: pitrilysin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 965 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF00675: Peptidase_M16" amino acids 54 to 190 (137 residues), 113.3 bits, see alignment E=2e-36 PF05193: Peptidase_M16_C" amino acids 214 to 392 (179 residues), 86 bits, see alignment E=6e-28 amino acids 680 to 856 (177 residues), 44.7 bits, see alignment E=2.8e-15 PF16187: Peptidase_M16_M" amino acids 399 to 663 (265 residues), 199.5 bits, see alignment E=1.2e-62 PF22456: PqqF-like_C_4" amino acids 777 to 875 (99 residues), 107.5 bits, see alignment E=6.7e-35

Best Hits

Swiss-Prot: 61% identical to PTRA_ECOL6: Protease 3 (ptrA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01407, protease III [EC: 3.4.24.55] (inferred from 95% identity to ddd:Dda3937_00856)

MetaCyc: 61% identical to protease 3 (Escherichia coli K-12 substr. MG1655)
Pitrilysin. [EC: 3.4.24.55]

Predicted SEED Role

"Protease III precursor (EC 3.4.24.55)" (EC 3.4.24.55)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XUD0 at UniProt or InterPro

Protein Sequence (965 amino acids)

>DZA65_RS05335 pitrilysin (Dickeya dianthicola ME23)
MRKSCIWLSALLLCFWFPISQAADGWQPLPETIRKSENDVRQYQAIRLDNGMTVLLVSDT
QATRSLAALALPVGSLDNPAQQPGLAHYLEHMLLMGSKRYPQADGLAEFLKMHGGSHNAS
TASYRTAFYLEVENDALQPAVDRLADAITEPLLDPVNADRERHAVNAELTMARARDGLRM
EQVEAETINPAHPGSRFAGGNLETLSDKPGSKLHDELVNFYQRYYSANLMKGVIYGKLPL
PDLAAIAASTFGRIANRQASVPPINAPVVTDEQRGLFIHYVPAQPRKQLKIEFRIDNNSQ
AFRCKTDTYISYLIGNRSQNTLSDWLQKQGLAESVRASVDPMSERDSGVFNISIDLTDKG
LEQQDNVIAGVFSYLEKLRKEGIQSRYFDEISRVLDIDFRYPSLSRDMRYVEWLADTMLR
LPVEYTLEGPYLADRFDPAAINARLSAMTPKNARIWVISPEQPHNKEAYFVGAPYQIDKI
GDARIALWQKTAQSLALSLPTPNPYIPDSFSLITADAGITHPKKVVDKPGLRVFYMPSRH
FASEPKADITLMLRNRIASDSARHQVLFALNDYLAGVALDALSYQASVGGISFSTGSDDG
LMMTASGYTQHLPALLLTLVEQYASFSSTEEQLEQAKSWYVEQLDAAEKAKAYEQAMFPI
KGLSSVPYSERSERRNLLKDITLQELMQYRKALLQQAAPEMLVVGNLEQDKVVSLSHSLR
DRLGCGGTEWWRGQSVSISQSQRATLQRSAGSTDSALAAVYIPAGYDEVQSAAYSKLLGQ
IVHPWFFNQLRTDEQLGYAVFATPVSVDRQWGIGFLLQSNSQQPAYLYQRYQDFFGKAEQ
RLSTMSAETFAQNKQGLINALSQPPQTLDEEAARLRGDLERENFAFDTRQQLIGQLASIS
SAQLTDFFRQALHPQGLAILSQVSGSANGKTDYARLSEWQSYASTSALQRTLTIKPVEQK
QTQTQ