Protein Info for DZA65_RS04885 in Dickeya dianthicola ME23

Annotation: RNA-directed DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 626 PF00078: RVT_1" amino acids 95 to 240 (146 residues), 88.6 bits, see alignment E=7.5e-29 PF01348: Intron_maturas2" amino acids 411 to 505 (95 residues), 35.5 bits, see alignment E=1.4e-12 PF21368: AI2M-like_HNH" amino acids 564 to 611 (48 residues), 68.3 bits, see alignment 6.3e-23

Best Hits

KEGG orthology group: None (inferred from 96% identity to dze:Dd1591_0690)

Predicted SEED Role

"Retron-type RNA-directed DNA polymerase (EC 2.7.7.49)" (EC 2.7.7.49)

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.49

Use Curated BLAST to search for 2.7.7.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XXF0 at UniProt or InterPro

Protein Sequence (626 amino acids)

>DZA65_RS04885 RNA-directed DNA polymerase (Dickeya dianthicola ME23)
MLSQRTLNALSGINKASIQGYKIKKLHKIMCSNKDLWAQAYANIYSNSGAITRGINNNTM
DEMSVDRIISLIHLIDSGSYIPKPCRRTHIPKDARKPNGKKRPLGIPTGDDKLTQEVMRM
LLEEIYEPVFSDWSYGFRPKRSCHSALKEIRNGWKGTKWVCDVDIKGYFDNIDHDLLLKF
LSKRIADNKFLALLKKLLKAGYLDNWRYFGTHSGTPQGGIISPILANVFLHELDEFMKNR
ITEFGKGGRRKPNPIYKRALQNRANRIKWIRQGFGASGMPADEQKIQKWKHEADELEKQL
RTLSSVIMDDTEFKRMRYVRYADDFLIGVIGSKGEAKKIMEEVVDFVESELHLEISKEKS
GIIDPKKGFTFLGYEIKTRRERKQVKCVVGLNTDGSKTHAVKRTITEHVHLGVPRERILK
LCSLRGYGNYDGGYGNSAGRPAMMHMSDYEIIGQYNAEMRGYANYYNLAPVLYLNKLHWI
WENSLYKTLAGKHRISRTKIARRLRQADGRYVYRENRNGKVYELEVFRLKNRSAPKHSSP
DILPNTHKYSGRTELLDRMAACQCEYCGTRTGPFEVHHVRKLKDIKSGTDNWKKLMIARN
RKTLVICRTCHVNLHRGTLPDRRSFV