Protein Info for DZA65_RS04865 in Dickeya dianthicola ME23

Annotation: DNA topoisomerase III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 674 TIGR01056: DNA topoisomerase III" amino acids 1 to 672 (672 residues), 810 bits, see alignment E=1e-247 PF01751: Toprim" amino acids 3 to 119 (117 residues), 53.4 bits, see alignment E=3.7e-18 PF01131: Topoisom_bac" amino acids 150 to 592 (443 residues), 357.4 bits, see alignment E=1.8e-110 PF01396: zf-C4_Topoisom" amino acids 620 to 648 (29 residues), 37.2 bits, see alignment (E = 3.1e-13)

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 64% identity to pwa:Pecwa_0618)

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster" (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4DE82 at UniProt or InterPro

Protein Sequence (674 amino acids)

>DZA65_RS04865 DNA topoisomerase III (Dickeya dianthicola ME23)
MRLFLCEKPSQGRDIAAVLGASKRGQGFLSGPDTVVTWAVGHLLENAMPELYGEQYGKPW
RLDVLPLLPQKWQMVVKKDTADQFAVITRLLKQSDEVVIATDADREGEVIARELLEYCRF
NGVVSRLWLSALDEASVRKALANIRPGNETENLYYAGLGRGRADWLIGMNISRLYTLKAR
ELGYDGVLSVGRVQTPALALVVNRDRLIASFVPKPYFQVNVKLESGGVVFPAEWVAAAAY
TDGERRCVNQAVAEQVRKLCLTVGKAVILDVEKKREKIPAPLAFSLGVLQQVCAERFGYS
PQQVLDTAQALYETHKATTYPRTDCGYLPESMLSEVPHVLSALQQSDPSLAPVIASIDPS
FRSRIWDDKKITAHHAIIPTPHKCNLAGMNEAERNVYTLIRTHYLAQFMPLLEMDVTRAS
FNIGGQLFRTSGRVTVVPGWKTLFGQAAASDSDERDDEAAAVSLPPLTKDAVCGIRGAEL
KALKTKPPQPYSFKTLIAAMMNAAGLVDDPALKKVLRDNAGLGTEATRAGILDVLMKRGF
LIKKGNHLHASDTACDLVDALPAALTQVGMTALWEQALDDIAAGKMTLDSFVDRQLRWVN
QLVEQGKQQAFTIRVPERPKCPLCGGRTRLRKGPNGTFYGCENYPTCKGIVDSGANNKRR
NRSGSSKSFNKKQN