Protein Info for DZA65_RS04535 in Dickeya dianthicola ME23

Annotation: NADPH-dependent 7-cyano-7-deazaguanine reductase QueF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 280 TIGR03138: queuine synthase" amino acids 10 to 280 (271 residues), 386.1 bits, see alignment E=5.4e-120 PF14819: QueF_N" amino acids 18 to 128 (111 residues), 153.6 bits, see alignment E=2.3e-49 PF14489: QueF" amino acids 194 to 267 (74 residues), 80.9 bits, see alignment E=6.7e-27

Best Hits

Swiss-Prot: 63% identical to QUEF_TOLAT: NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) from Tolumonas auensis (strain DSM 9187 / TA4)

KEGG orthology group: K06879, 7-cyano-7-deazaguanine reductase [EC: 1.7.1.13] (inferred from 98% identity to ddd:Dda3937_01462)

MetaCyc: 59% identical to 7-cyano-7-deazaguanine reductase (Escherichia coli K-12 substr. MG1655)
PreQ(1) synthase. [EC: 1.7.1.13]

Predicted SEED Role

"NADPH dependent preQ0 reductase (EC 1.7.1.13)" (EC 1.7.1.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XVV3 at UniProt or InterPro

Protein Sequence (280 amino acids)

>DZA65_RS04535 NADPH-dependent 7-cyano-7-deazaguanine reductase QueF (Dickeya dianthicola ME23)
MHITENDSITHLGVRSSYPDKYDPALLEALPRARGRDLVGLTGPDLPFDGYDLWTAFELS
WLNHKGKPLVGIAEFIIPASSENLIESKSFKLYLNSFNQTRFRDIGEVHATLIKDLSAAA
NGDVKVTLYPGLTGYPAQIDTLPGINIDELDIEVNDYGFNPDYLQHAVRDNAPVVAETLC
SNLLKSNCLVTYQPDWGSVVIKYEGRQIDREALLRYLISFRQHNEFHEQCVERIFNDLKR
YCRPEKLTVFARYTRRGGLDINPFRSDFETQAATGRLVRQ