Protein Info for DZA65_RS04515 in Dickeya dianthicola ME23

Annotation: multidrug efflux MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 47 to 66 (20 residues), see Phobius details amino acids 78 to 96 (19 residues), see Phobius details amino acids 102 to 124 (23 residues), see Phobius details amino acids 134 to 155 (22 residues), see Phobius details amino acids 164 to 185 (22 residues), see Phobius details amino acids 206 to 227 (22 residues), see Phobius details amino acids 248 to 270 (23 residues), see Phobius details amino acids 282 to 301 (20 residues), see Phobius details amino acids 307 to 329 (23 residues), see Phobius details amino acids 341 to 362 (22 residues), see Phobius details amino acids 370 to 391 (22 residues), see Phobius details PF07690: MFS_1" amino acids 12 to 222 (211 residues), 112.3 bits, see alignment E=3.7e-36 amino acids 216 to 392 (177 residues), 89.1 bits, see alignment E=4.1e-29 PF12832: MFS_1_like" amino acids 16 to 370 (355 residues), 36.3 bits, see alignment E=5.4e-13 PF00083: Sugar_tr" amino acids 40 to 125 (86 residues), 34.8 bits, see alignment E=1.4e-12

Best Hits

Swiss-Prot: 64% identical to MDTG_KLEP3: Multidrug resistance protein MdtG (mdtG) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K08161, MFS transporter, DHA1 family, multidrug resistance protein (inferred from 95% identity to dze:Dd1591_3239)

MetaCyc: 61% identical to efflux pump MdtG (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-342; TRANS-RXN0-589

Predicted SEED Role

"Multidrug-efflux transporter, major facilitator superfamily (MFS) (TC 2.A.1)" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CRJ1 at UniProt or InterPro

Protein Sequence (397 amino acids)

>DZA65_RS04515 multidrug efflux MFS transporter (Dickeya dianthicola ME23)
METWKLNLFSAWLGCFFTGMAMSQILPFLPLYIEHLGVSDHTALNLWSGLVFSSSFLVSA
IVAPLWGSLADRKGRKLMLLRAALGMSLVMALQGMATNVWQLFILRTLMGLTSGYIPNAM
ALIASQVPRSRSGWALGTLSTGQVAGVLIGPLLGGFMADTIGLRMVFFITSAMLFICFLI
TLFAIRENAVTISKKAQLSGRAVFASLPYPLLIISLCFTTMMIQMANGSISPILTLFIRD
LSPNIDNIAFISGVIAAVPGMSALLSASHLGRLGDSIGSQRVLIVALFLCALLLFLMSAV
QSSVQLGILRFALGFADGALMPAVQALLVKYSSQQVTGRIFGYNQSCMYLGNVVGPLIGS
TVSASLGYRWVFLVTALLVLINGIQLMVSFARLPKKV