Protein Info for DZA65_RS04460 in Dickeya dianthicola ME23

Annotation: cytochrome c nitrite reductase subunit NrfD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 318 transmembrane" amino acids 16 to 36 (21 residues), see Phobius details amino acids 49 to 72 (24 residues), see Phobius details amino acids 91 to 113 (23 residues), see Phobius details amino acids 152 to 170 (19 residues), see Phobius details amino acids 181 to 203 (23 residues), see Phobius details amino acids 224 to 243 (20 residues), see Phobius details amino acids 255 to 277 (23 residues), see Phobius details amino acids 289 to 312 (24 residues), see Phobius details TIGR03148: cytochrome c nitrite reductase, NrfD subunit" amino acids 4 to 318 (315 residues), 400.5 bits, see alignment E=2.6e-124 PF03916: NrfD" amino acids 4 to 318 (315 residues), 277.1 bits, see alignment E=2.5e-86 PF14589: NrfD_2" amino acids 11 to 316 (306 residues), 46.7 bits, see alignment E=3.7e-16

Best Hits

KEGG orthology group: K04015, formate-dependent nitrate reductase complex, transmembrane protein (inferred from 93% identity to ddd:Dda3937_01446)

Predicted SEED Role

"NrfD protein" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CVH9 at UniProt or InterPro

Protein Sequence (318 amino acids)

>DZA65_RS04460 cytochrome c nitrite reductase subunit NrfD (Dickeya dianthicola ME23)
MHDAFHFPSLVWDWPIAVYLLLVGISSGMMVLTLLFRRYLPAENQHTNPLITATAIVAPL
SVVVGLVILIFHLARPVTFWYLMIFYNPSSIMSLGVMLFQVYMLVMFLWLASLYQQPLLA
WSERHLPGALHGLLNRVIALVSRGMRPVENGLLVLALLLGCYTGFLLSALKSFPLLNNPL
LPVLFLVSGVTSGIAVLMMVGASARSHRDCPQTLHLLHALEKPVVLVELFLLFAFFVGLL
LGGGQKAVAAQVALSGFWGGVFWIGIIGIGMLAPLAAGSQPVMRRHVAYLPRIAAGLTLL
GVFLLRLFVLYAGQMTVA