Protein Info for DZA65_RS04445 in Dickeya dianthicola ME23

Annotation: FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 523 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF07992: Pyr_redox_2" amino acids 51 to 238 (188 residues), 36 bits, see alignment E=1.6e-12 PF01266: DAO" amino acids 51 to 240 (190 residues), 42.3 bits, see alignment E=2.3e-14 PF01134: GIDA" amino acids 51 to 243 (193 residues), 25.4 bits, see alignment E=2.2e-09 PF00890: FAD_binding_2" amino acids 51 to 492 (442 residues), 208.2 bits, see alignment E=8.1e-65 PF12831: FAD_oxidored" amino acids 51 to 234 (184 residues), 43.3 bits, see alignment E=9.4e-15 PF13450: NAD_binding_8" amino acids 54 to 87 (34 residues), 22.9 bits, see alignment (E = 2.6e-08)

Best Hits

KEGG orthology group: K00244, fumarate reductase flavoprotein subunit [EC: 1.3.99.1] (inferred from 97% identity to ddd:Dda3937_01443)

Predicted SEED Role

"Fumarate reductase flavoprotein subunit (EC 1.3.99.1)" in subsystem Succinate dehydrogenase (EC 1.3.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.1

Use Curated BLAST to search for 1.3.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XX82 at UniProt or InterPro

Protein Sequence (523 amino acids)

>DZA65_RS04445 FAD-dependent oxidoreductase (Dickeya dianthicola ME23)
MAQYRKKLLAALCLSVLGITSAQASGPAESTAEPAKSAEPAKPVELPKSADVVIIGAGAA
GTAATMAAAEKGASVVLLEKQGAVGGTGNFAEGIFAANSAMQKRQGIVVTPDMAFKTIME
YSHWMANPFVVRAFVNRSADTIEWVKSKGIKFEYIGPGGPGGMLTWHVIDGPGHGRHLIK
TFHEQFKTMKVTTLVKTAGKDLVMKDGKVAGVIAEGSDGKPFQIDAKAVIVATGGYANNK
EMLQKYVAVPDTIMVGNVGKDGDGIKMAWKAGAKEEGMGVVQSYRPGLPDYAPNSQLLAA
ARQPYLWVDKTGHRFTDESIVIIWPHAGNALAKAGGVMYSVFDEDARKHFVNDGIDVPIG
EWVIANTKLVKFDDEFTKESQKNRGFVFKAATLDELAKQMGVDASVLKHTVDENNRFAAQ
KRDEVFNKNMDYLRPMKTGPFYAVKMLPAALGTLGGVKINEKMEAVSPAGNAVPGLYVTG
NDAAGMYGDTYDLLLGGGTFGFALNSGRMAGENALDYLHFTKK